Landsperger W J, Kelly-Wintenberg K D, Montie T C, Knight L S, Hansen M B, Huntenburg C C, Schneidkraut M J
Immunotherapy Division, Baxter Healthcare Corporation, Duarte, California.
Infect Immun. 1994 Nov;62(11):4825-30. doi: 10.1128/iai.62.11.4825-4830.1994.
Two human monoclonal antibodies, directed against the type a and type b flagellar proteins of Pseudomonas aeruginosa, inhibited bacterial motility in vitro specifically and in a concentration-dependent manner. In order to determine if this decreased bacterial motility was associated with a decreased pathogenicity, the ability of these human antiflagellar monoclonal antibodies to attenuate P. aeruginosa-induced pneumonia in the rat was assessed. Incubation of P. aeruginosa with a 1:1 mixture of the human antiflagellar monoclonal antibodies prior to pulmonary instillation significantly (P < 0.05) ameliorated the bacterium-induced decrease in arterial blood oxygen pressure, blunted the increase in respiratory rate, and markedly reduced the area of pulmonary inflammation. Similarly, intravenous administration of the human antiflagellar monoclonal antibodies 1 h after pulmonary instillation of the bacteria also reduced the in vivo pathogenicity of P. aeruginosa. Therefore, human antiflagellar monoclonal antibodies can decrease the in vitro motility of P. aeruginosa and can reduce its in vivo pathogenicity when administered either before or after bacterial challenge.
两种针对铜绿假单胞菌a型和b型鞭毛蛋白的人源单克隆抗体，在体外能特异性地、以浓度依赖的方式抑制细菌运动。为了确定这种细菌运动性降低是否与致病性降低相关，评估了这些人源抗鞭毛单克隆抗体减轻大鼠铜绿假单胞菌诱导的肺炎的能力。在肺部灌洗前，将铜绿假单胞菌与人源抗鞭毛单克隆抗体以1:1的混合物孵育，显著（P<0.05）改善了细菌诱导的动脉血氧压降低，减弱了呼吸频率的增加，并显著减少了肺部炎症面积。同样，在肺部灌洗细菌1小时后静脉注射人源抗鞭毛单克隆抗体，也降低了铜绿假单胞菌的体内致病性。因此，人源抗鞭毛单克隆抗体可降低铜绿假单胞菌的体外运动性，并且在细菌攻击前或攻击后给药时均可降低其体内致病性。
