Ansorg R
Zentralbl Bakteriol Orig A. 1978 Nov;242(2):228-38.
Serogrouping of P. aeruginosa on the basis of heat-stable somatic (O-)agglutinogens is well established, but H-typing on the basis of heat-labile agglutinogens is not accepted generally because of uncertain flagella specificity. In contrast to the agglutination reaction, the indirect fluorescent antibody technique allows the differentiation of soma and flagellum of P. aeruginosa cells at the morphological and serological level simultaneously, and thereby the specific analysis of flagellar (H-)antigens, even with OH-antisera. Using this technique the complex flagella-antigen a with the partial antigens a0, a1, a2, a3, a4 and the uniform flagella-antigen b are differentiated by cross absorption experiments. As the partial factors a1-a4 are independent determinants, a flagellar antigenic schema containing 17 H-types is devised. Type strains, immunization and absorption procedures for preparing diagnostic antisera are described. O-group and H-type show free combination. Completing the extended O antigenic schema of Habs (slide agglutination) with the flagella specific H antigenic schema (indirect fluorescent antibody technique), a detailed OH typing system of P. aeruginosa is obtained, by which clinical isolates are subdivided into 99 serovars.
基于热稳定菌体（O-）凝集原对铜绿假单胞菌进行血清学分型已得到充分确立，但基于热不稳定凝集原的H分型由于鞭毛特异性不确定而未被普遍接受。与凝集反应不同，间接荧光抗体技术能够在形态学和血清学水平上同时区分铜绿假单胞菌细胞的菌体和鞭毛，从而即使使用O+H抗血清也能对鞭毛（H-）抗原进行特异性分析。利用该技术，通过交叉吸收实验区分了具有部分抗原a0、a1、a2、a3、a4的复合鞭毛抗原a和单一鞭毛抗原b。由于部分因子a1 - a4是独立的决定簇，因此设计了一个包含17种H型的鞭毛抗原模式。描述了用于制备诊断抗血清的标准菌株、免疫和吸收程序。O群和H型表现出自由组合。用鞭毛特异性H抗原模式（间接荧光抗体技术）完善Habs扩展的O抗原模式（玻片凝集），得到了一个详细的铜绿假单胞菌O+H分型系统，通过该系统可将临床分离株细分为99个血清型。
