Semenova-Tian-Shanskaia A G
Arkh Anat Gistol Embriol. 1976 Oct;71(10):52-8.
The work was carried out in 23 rat embryos from 9,5 to the 11th day of development. In 9,5-day embryos the primary sex cells are localized in the mesenchyma of the allantois and in the intestinal entoderm. Later they migrate either with the blood flow or on the surface of cellular layers towards gonad germs which are reached by the 11th day of the intrauterine development. In the course of this process there occur structural and cytochemical changes in gonocyte nuclei. The nucleolus is replaced to the periphery of the nucleus, around it there appears a rim intensively stained with methylene green. Chromatin has a shape of thin threads. These changes of the nuclei seem to be associated with an increased synthesis of r-DNA and hence with the synthesis of substances having the role to prevent the somatic differentiation of primary sex cells in the course of migration.
该研究在23个发育9.5至11天的大鼠胚胎中进行。在9.5天的胚胎中，原始生殖细胞位于尿囊的间充质和肠内胚层中。随后，它们要么随血流迁移，要么在细胞层表面朝着性腺原基迁移，在子宫内发育的第11天到达性腺原基。在这个过程中，生殖细胞核会发生结构和细胞化学变化。核仁移至细胞核周边，其周围出现一条被亚甲基绿强烈染色的边缘。染色质呈细线状。细胞核的这些变化似乎与r-DNA合成增加有关，因此也与在迁移过程中具有阻止原始生殖细胞体细胞分化作用的物质合成有关。
