Kemper C H, Peters P W
Department of Teratology, National Institute of Public Health and Environmental Hygiene, Bilthoven, The Netherlands.
Teratology. 1987 Aug;36(1):117-24. doi: 10.1002/tera.1420360115.
Information about early primordial germ cell (PGC) formation and migration in rats is lacking. In utero developed and in vitro cultivated whole rat embryos were studied on days 10-13 postcoitum (p.c.). The development of the PGCs was investigated in serial sections stained for alkaline phosphatase activity. On postcoital day 10, PGCs were found in the invaginating visceral yolk sac endoderm and at the base of the allantois. At day 11 p.c. PGCs were mostly found in the ventral and lateral gut wall or in the mesenchyme between the gut and the future genital ridges. At day 12 p.c. most of the PGCs (94%) could be localised in the mesenchyme or in the future genital ridges. On postcoital day 13 almost all PGCs had reached the now-well-developed genital ridges. Quantitative measurements showed an increase in the number of PGCs from 84 at day 10 p.c. up to 2,768 at day 13 p.c. Only slight differences were found between in vivo and in vitro embryos with respect to the number of PGCs and their developmental pattern. The in vitro culture of whole rat embryos enables the discrimination between the effects of indirect (maternal) and direct action of PGC-toxic agents.
关于大鼠早期原始生殖细胞（PGC）形成和迁移的信息尚缺。研究了交配后（p.c.）第10 - 13天子宫内发育和体外培养的全胚大鼠。通过对碱性磷酸酶活性染色的连续切片研究PGC的发育。在交配后第10天，在内陷的脏壁卵黄囊内胚层和尿囊基部发现了PGC。在交配后第11天，PGC大多见于腹侧和外侧肠壁或肠与未来生殖嵴之间的间充质中。在交配后第12天，大多数PGC（94%）可定位于间充质或未来生殖嵴中。在交配后第13天，几乎所有PGC都已到达此时发育良好的生殖嵴。定量测量显示PGC数量从交配后第10天的84个增加到交配后第13天的2768个。在PGC数量及其发育模式方面，体内和体外胚胎之间仅发现细微差异。全胚大鼠的体外培养能够区分PGC毒性剂的间接（母体）作用和直接作用。
