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[用小角X射线散射法研究噬菌体DD7的结构]

[Study of the structure of bacteriophage DD7 by the small angle of dispersion x-ray method].

作者信息

Boiarintseva A K, Davydova M A, Dembo A T

出版信息

Vopr Virusol. 1976 Nov-Dec(6):731-4.

PMID:1030906
Abstract

The method of roentgen small angle dispersion was used for the study of the structure of bacteriophage DD7. According to the results, phage DD7 is a homogeneous icosahedral particle (with the edge of 410-415 A) with a round cylindrical tail (1700-1750 A in length, 110-120 A in diameter). The lineal sizes of the head exceed those determined by electron microscope approximately by 25%. The thickness of the protein coat of the phage is 25-30 A, that is, 2 times less than that of phages Cd and PB-2. This confirms the assumption that the lipid-containing portion of the phage coat is eliminated by centrifugation in cesium chloride density gradient.

摘要

采用X射线小角散射法研究噬菌体DD7的结构。结果表明,噬菌体DD7是一种均匀的二十面体颗粒(边长为410 - 415埃),带有一个圆柱形尾部(长度为1700 - 1750埃,直径为110 - 120埃)。头部的线性尺寸比电子显微镜测定的尺寸大约大25%。噬菌体蛋白质外壳的厚度为25 - 30埃,即比噬菌体Cd和PB - 2的厚度小2倍。这证实了关于通过氯化铯密度梯度离心去除噬菌体外壳中含脂部分的假设。

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