Rontó G, Agamalyan M M, Drabkin G M, Feigin L A, Lvov Y M
Biophys J. 1983 Sep;43(3):309-14. doi: 10.1016/S0006-3495(83)84354-9.
Small-angle x-ray and neutron scattering techniques were applied to bacteriophage T7 solutions at different scattering densities. Scattering curves determined under a variety of experimental conditions were used to derive a set of parameters characterizing the shape, size, and weight of the whole phage particle and of its DNA and protein components. The T7 head has an icosahedral shape with an edge of 37.7 +/- 0.5 nm, a volume of (12.0 +/- 1.0) x 10(4) nm3, and a small tail amounting to 6--7% of the head volume. The intraphage DNA region is most probably a hollow sphere. The best fit to the data was obtained with a model in which the hollow sphere filled with a protein core with a diameter of 24 nm. The average degree of swelling (i.e., the ratio of the hydrated to the dry volume) of the particle is 2.3; the degree of swelling of the DNA component is higher, 3.2, and that of the protein part is lower, 1.2.
小角X射线和中子散射技术被应用于不同散射密度的噬菌体T7溶液。在各种实验条件下测定的散射曲线被用于推导一组表征整个噬菌体颗粒及其DNA和蛋白质成分的形状、大小和重量的参数。T7头部呈二十面体形状，边长为37.7±0.5纳米，体积为(12.0±1.0)×10⁴立方纳米，还有一条小尾巴，占头部体积的6 - 7%。噬菌体内部的DNA区域很可能是一个空心球体。用一个模型能最好地拟合数据，该模型中空心球体填充着一个直径为24纳米的蛋白质核心。颗粒的平均膨胀程度（即水合体积与干燥体积之比）为2.3；DNA成分的膨胀程度较高，为3.2，而蛋白质部分的膨胀程度较低，为1.2。
