Singer R, Landau B, Joshua H, Zukerman Z, Pick I, Sigienriech E, Chowers I
Acta Eur Fertil. 1976 Dec;7(4):281-4.
Protein concentrations of seminal plasma and spermatozoa were estimated in human normospermic, oligospermic and azoospermic semens. The protein content of seminal plasma ranged from 20 to 60 mg per ml semen and was found to be unrelated to sperm counts. Suspended sediments of azoospermic specimens were found to contain on the average 570+/-86 microng protein per ml semen. This value is about four times higher than in severe oligospermic specimens (0.2 X 10(6) -6.5 X 10(6) cells per ml semen). It is suggested that in azoospermic disorders seminiferous tubules secrete abnormally high amounts of proteinacous material which cannot be eliminated by washings. In addition, destruction of immature cells may contribute to increased protein conten in these specimens. Protein determinations in washed spermatozoa showed a negative relationship between the amount of protein calculated per 10(6) cells and original sperm counts. This trend demonstrates an altered protein metabolism in the spermatozoa of oligospermic specimens which might be responsible for fertility incompetence.
对人类正常精子、少精子和无精子精液中的精浆和精子的蛋白质浓度进行了估算。精浆的蛋白质含量为每毫升精液20至60毫克，且发现与精子计数无关。发现无精子标本的悬浮沉淀物平均每毫升精液含有570±86微克蛋白质。该值约为严重少精子标本（每毫升精液0.2×10⁶ - 6.5×10⁶个细胞）的四倍。有人提出，在无精子症中，生精小管分泌异常大量的蛋白质物质，这些物质无法通过冲洗消除。此外，未成熟细胞的破坏可能导致这些标本中蛋白质含量增加。对洗涤后的精子进行蛋白质测定显示，每10⁶个细胞计算出的蛋白质量与原始精子计数之间呈负相关。这种趋势表明少精子标本的精子中蛋白质代谢发生了改变，这可能是导致生育能力不足的原因。
