Limpaseni T, Tanphaichitr V, Tanphaichitr N
Int J Fertil. 1980;25(1):57-61.
Activities of carnitine acetyltransferase (CAT) were determined in fertile (greater than 40 X 10(6) sperm/ml) and oligospermic infertile semen (less than 15 X 10(6) sperm/ml). CAT specific activities (unit/10(6) sperm) appear to be similar in both kinds of semen i.e., 0.020 +/- 0.002 and 0.013 +/- 0.001 for fertile and oligospermic infertile sperm, respectively. When CAT activities were corrected as unit/mg protein and unit/mg DNA, the values in the fertile sperm were 0.130 +/- 0.010 and 8.97 +/- 0.50, respectively, while in the infertile sperm the values were 0.016 +/- 0.002 and 2.72 +/- 0.14. The lower value in the oligospermic infertile sperm is due to the much higher amount of these macromolecules in infertile sperm. On the other hand, CAT activities and the amount of protein appear to be similar in both fertile and oligospermic seminal plasma. Our results, therefore, suggest that the lower level of CAT in infertile semen is due to its lower sperm density.
测定了正常生育男性（精子浓度大于40×10⁶/ml）和少精子症不育男性（精子浓度小于15×10⁶/ml）精液中肉碱乙酰转移酶（CAT）的活性。两种精液中CAT的比活性（单位/10⁶精子）似乎相似，即正常生育男性精子和少精子症不育男性精子的比活性分别为0.020±0.002和0.013±0.001。当将CAT活性校正为单位/毫克蛋白质和单位/毫克DNA时，正常生育男性精子中的值分别为0.130±0.010和8.97±0.50，而不育男性精子中的值分别为0.016±0.002和2.72±0.14。少精子症不育男性精子中该值较低是由于不育男性精子中这些大分子的含量高得多。另一方面，正常生育男性和少精子症男性精浆中的CAT活性和蛋白质含量似乎相似。因此，我们的结果表明，不育精液中CAT水平较低是由于精子密度较低。
