Gerrard J M, Butler A M, Peterson D A, White J G
Prostaglandins Med. 1978 Nov;1(5):387-96. doi: 10.1016/0161-4630(78)90125-8.
A platelet membrane fraction which actively sequesters calcium in the presence of ATP was prepared and the influence of phosphatidic acid evaluated. At 10--60 micrograms/ml phosphatidic acid caused a concentration dependent release of calcium from the membrane fraction. The calcium was released from inside the vesicles, since release occurred in the presence of EGTA used to bind calcium outside the membrane vesicles. Aspirin failed to inhibit release of calcium by phosphatidic acid. Our results may explain, in part, the prostaglandin and thromboxane independent calcium release which occurs in response to certain aggregating agents. Thus, phosphatidic acid, or a metabolite, may have an important role intracellularly in platelets in promoting calcium movement.
制备了一种在ATP存在下能主动螯合钙的血小板膜组分，并评估了磷脂酸的影响。在10 - 60微克/毫升时，磷脂酸导致膜组分中钙的浓度依赖性释放。钙从囊泡内部释放，因为在用于结合膜囊泡外部钙的EGTA存在下发生了释放。阿司匹林未能抑制磷脂酸引起的钙释放。我们的结果可能部分解释了响应某些聚集剂而发生的不依赖前列腺素和血栓素的钙释放。因此，磷脂酸或其代谢产物可能在细胞内对血小板促进钙移动起重要作用。
