Diversé-Pierluissi M A, Fischer T, Jordan J D, Schiff M, Ortiz D F, Farquhar M G, De Vries L
Department of Physiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
J Biol Chem. 1999 May 14;274(20):14490-4. doi: 10.1074/jbc.274.20.14490.
Norepinephrine inhibits omega-conotoxin GVIA-sensitive presynaptic Ca2+ channels in chick dorsal root ganglion neurons through two pathways, one mediated by Go and the other by Gi. These pathways desensitize at different rates. We have found that recombinant Galpha interacting protein (GAIP) and regulators of G protein signaling (RGS)4 selectively accelerate the rate of desensitization of Go- and Gi-mediated pathways, respectively. Blockade of endogenous RGS proteins using antibodies raised against Galpha interacting protein and RGS4 slows the rate of desensitization of these pathways in a selective manner. These results demonstrate that different RGS proteins may interact with Gi and Go selectively, giving rise to distinct time courses of transmitter-mediated effects.
去甲肾上腺素通过两条途径抑制鸡背根神经节神经元中对ω-芋螺毒素GVIA敏感的突触前Ca2+通道，一条由Go介导，另一条由Gi介导。这些途径以不同的速率脱敏。我们发现重组Gα相互作用蛋白（GAIP）和G蛋白信号调节剂（RGS）4分别选择性地加速Go和Gi介导途径的脱敏速率。使用针对Gα相互作用蛋白和RGS4产生的抗体阻断内源性RGS蛋白，以选择性方式减慢这些途径的脱敏速率。这些结果表明，不同的RGS蛋白可能分别与Gi和Go选择性相互作用，从而产生递质介导效应的不同时间进程。
