Ueki N, Seki N, Yano K, Saito T, Masuho Y, Muramatsu M
Helix Research Institute, Chiba, Japan.
J Hum Genet. 1999;44(3):193-6. doi: 10.1007/s100380050141.
Mouse PIAS3 (protein inhibitor of activated STAT3) is a specific inhibitor of STAT3 that downregulates its signaling pathway. Here we report the isolation and chromosome mapping of the human PIAS3 gene. Human PIAS3 cDNA encoded a predicted protein of 619 aa which has 83% overall amino acid identity to the mouse counterpart. Based on polymerase chain reaction assisted analysis of a human/rodent mono-chromosomal hybrid cell panel and a radiation hybrid mapping panel, the human PIAS3 gene was mapped to the chromosome 1q21 region. Mapping of a crucial gene in modulating the STAT3 signaling pathway may provide new clues to the understanding of malignancies or genetic disorders caused by this chromosome region.
小鼠PIAS3（信号转导和转录激活因子3激活蛋白抑制剂）是信号转导和转录激活因子3的特异性抑制剂，可下调其信号通路。在此，我们报告人类PIAS3基因的分离和染色体定位。人类PIAS3 cDNA编码一个预测的含619个氨基酸的蛋白质，该蛋白质与小鼠对应物的整体氨基酸同一性为83%。基于对人/啮齿动物单染色体杂交细胞系和辐射杂种定位板的聚合酶链反应辅助分析，将人类PIAS3基因定位于1q21染色体区域。对调节信号转导和转录激活因子3信号通路的关键基因进行定位，可能为理解由该染色体区域引起的恶性肿瘤或遗传疾病提供新线索。
