Seki N, Ueki N, Yano K, Saito T, Masuho Y, Muramatsu M
Genome Research Group, National Institute Radiological Sciences, Chiba, Japan.
J Hum Genet. 2000;45(1):31-7. doi: 10.1007/s100380070040.
A-kinase anchoring protein 95 (AKAP95) is a nuclear protein which binds to the regulatory subunit (RII) of cyclic adenosine monophosphate (cAMP)-dependent protein kinase (PKA) and to DNA. A novel nuclear human gene which shares sequence homology with the human AKAP95 gene was identified by a nuclear transportation trap method. By polymerase chain reaction (PCR)-based analysis with both a human/rodent monochromosomal hybrid cell panel and a radiation hybrid panel, the gene was mapped to the chromosome 19p13.11-p13.12 region between markers WI-4669 and CHLC.GATA27C12. Furthermore, alignment with genomic sequences revealed that the gene and human AKAP95 resided tandemly only approximately 250 bp apart from each other. We designated this gene as neighbor of AKAP95 (NAKAP95). The exon-intron structure of NAKAP95 and AKAP95 was conserved, indicating that they may have evolved by gene duplication. The predicted protein product of the NAKAP95 gene consists of 646 amino acid residues, and NAKAP95 and AKAP95 had an overall 40% similarity, both having a potential nuclear localizing signal and two C2H2 type zinc finger motifs. The putative RII binding motif in AKAP95 was not conserved in NAKAP95. A reverse transcription coupled (RT)-PCR experiment revealed that the NAKAP95 gene was transcribed ubiquitously in various human tissues.
A激酶锚定蛋白95（AKAP95）是一种核蛋白，它可与环磷酸腺苷（cAMP）依赖性蛋白激酶（PKA）的调节亚基（RII）以及DNA结合。通过核转运捕获法鉴定出了一个与人类AKAP95基因具有序列同源性的新型人类核基因。利用基于聚合酶链反应（PCR）的分析方法，对人类/啮齿动物单染色体杂交细胞系和辐射杂种细胞系进行检测，该基因被定位到19号染色体p13.11 - p13.12区域，位于标记WI - 4669和CHLC.GATA27C12之间。此外，与基因组序列比对发现，该基因与人类AKAP95基因串联排列，彼此间距仅约250 bp。我们将此基因命名为AKAP95的邻居（NAKAP95）。NAKAP95和AKAP95的外显子 - 内含子结构保守，表明它们可能通过基因复制进化而来。NAKAP95基因预测的蛋白质产物由646个氨基酸残基组成，NAKAP95与AKAP95总体相似度为40%，二者均具有潜在的核定位信号和两个C2H2型锌指基序。AKAP95中假定的RII结合基序在NAKAP95中并不保守。逆转录偶联（RT）-PCR实验表明，NAKAP95基因在各种人类组织中均有广泛转录。
