Nishiwaki T, Daigo Y, Kawasoe T, Nagasawa Y, Ishiguro H, Fujita M, Furukawa Y, Nakamura Y
Laboratory of Molecular Medicine, Institute of Medical Science, University of Tokyo, Japan.
J Hum Genet. 1999;44(3):197-202. doi: 10.1007/s100380050142.
We have isolated a human cDNA encoding a protein of 1288 amino acids that shows 77% identity in amino acid sequence to XCAP-C, Xenopus chromosome-associated polypeptide-C, belonging to the family of structural maintenance of chromosomes (SMC), which is known to play a crucial role in the proper condensation and segregation of mitotic chromosomes. In particular, an almost 200-amino-acid domain in the N-terminal, including an NTP-binding motif and that in the C-terminal domain, including a DA-box, were well conserved and showed 95% identity between human and frog, indicating that these two domains are functionally very important. The transcript of this gene was expressed ubiquitously in various human tissues, but thymus, testis, and colon seemed to express this gene more abundantly. We also determined its chromosomal location at 3q26.1 by fluorescence in situ hybridization.
我们分离出了一个人类cDNA，它编码一种由1288个氨基酸组成的蛋白质，该蛋白质的氨基酸序列与非洲爪蟾染色体相关多肽C（XCAP-C）有77%的同一性，XCAP-C属于染色体结构维持（SMC）家族，已知在有丝分裂染色体的正确凝聚和分离中起关键作用。特别是，N端一个近200个氨基酸的结构域，包括一个NTP结合基序，以及C端结构域中的一个DA框，都高度保守，人和蛙之间的同一性为95%，这表明这两个结构域在功能上非常重要。该基因的转录本在各种人类组织中普遍表达，但胸腺、睾丸和结肠似乎表达更为丰富。我们还通过荧光原位杂交确定了它在染色体上的位置为3q26.1。
