Takeda S, Fujiwara T, Shimizu F, Kawai A, Shinomiya K, Okuno S, Ozaki K, Katagiri T, Shimada Y, Nagata M, Watanabe T, Takaichi A, Kuga Y, Suzuki M, Hishigaki H, Takahashi E, Shin S, Nakamura Y, Hirai Y
Otsuka GEN Research Institute, Tokushima 771-01, Japan.
Cytogenet Cell Genet. 1997;76(1-2):87-93. doi: 10.1159/000134521.
From a human fetal-brain cDNA library, we isolated and characterized a novel gene (KPNA3) encoding a protein highly homologous to certain nuclear transport proteins of Xenopus and human. The complete cDNA clone, designated karyopherin alpha 3, contained an open reading frame of 1,563 nucleotides encoding 521 amino acids. The predicted amino acid sequence showed 48%, 45% and 48% identity with Xenopus importin, yeast SRP1 and human RCH1, respectively. The similarities among these proteins suggest that karyopherin alpha 3 may be involved in the nuclear transport system. Eight repeats of the arm motif were well conserved among these proteins. The N-terminal region of the predicted karyopherin alpha 3 product was highly basic and the C-terminal region was strongly acidic. A 4.3-kb transcript was expressed in all adult human tissues examined by Northern blotting. The cDNA clone was assigned to chromosome band 13q14.3 by fluorescence in situ hybridization.
我们从一个人类胎儿脑cDNA文库中分离并鉴定了一个新基因（KPNA3），该基因编码一种与非洲爪蟾和人类的某些核转运蛋白高度同源的蛋白质。完整的cDNA克隆，命名为核转运蛋白α3，包含一个1563个核苷酸的开放阅读框，编码521个氨基酸。预测的氨基酸序列与非洲爪蟾输入蛋白、酵母SRP1和人类RCH1的同一性分别为48%、45%和48%。这些蛋白质之间的相似性表明核转运蛋白α3可能参与核转运系统。这些蛋白质中臂基序的八个重复序列高度保守。预测的核转运蛋白α3产物的N端区域高度碱性，C端区域则呈强酸性。通过Northern印迹法检测，在所有检测的成人组织中均表达了一个4.3kb的转录本。通过荧光原位杂交将该cDNA克隆定位到染色体带13q14.3。
