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非同源DNA末端连接的生物化学及生物学意义：多细胞真核生物中的一种重要修复过程

The biochemistry and biological significance of nonhomologous DNA end joining: an essential repair process in multicellular eukaryotes.

作者信息

Lieber M R

机构信息

Norris Comprehensive Cancer Center, Department of Pathology, Los Angeles, CA 90033, USA.

出版信息

Genes Cells. 1999 Feb;4(2):77-85. doi: 10.1046/j.1365-2443.1999.00245.x.

DOI:10.1046/j.1365-2443.1999.00245.x
PMID:10320474
Abstract

Recent progress over the past year has provided new insights into the proteins involved in nonhomologous end joining. The assembly of Ku and DNA-dependent protein kinase at DNA ends is now understood in greater detail. Murine genetic knockouts for DNA ligase IV and XRCC4 are embryonic lethal, indicating that nonhomologous end joining is essential for viability. Interestingly, neurones, in addition to lymphocytes, are particularly vulnerable to an absence of NHEJ.

摘要

过去一年的最新进展为参与非同源末端连接的蛋白质提供了新的见解。现在对Ku和DNA依赖性蛋白激酶在DNA末端的组装有了更详细的了解。DNA连接酶IV和XRCC4的小鼠基因敲除是胚胎致死性的，这表明非同源末端连接对生存能力至关重要。有趣的是，除淋巴细胞外，神经元对缺乏非同源末端连接尤为敏感。

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