Zheng Z L, Travagli R A, Kreulen D L
Department of Physiology, Michigan State University, East Lansing, MI 48824-1317, USA.
Brain Res. 1999 May 8;827(1-2):113-21. doi: 10.1016/s0006-8993(99)01313-x.
The purpose of this study was to determine whether there is a specific organization of the primary sensory innervation on to identified vascular neurons in the inferior mesenteric ganglion (IMG) in guinea-pig. Retrograde tracers were placed intraluminally in inferior mesenteric artery (IMA) or inferior mesenteric vein (IMV) in vitro to identify ganglionic neurons as arterial, venous or unlabeled neurons. The distribution of primary sensory nerve fibers containing calcitonin gene-related peptide (CGRP), neuronal nitric oxide synthase (NOS) and substance P immunoreactivity (SP-IR) was compared before and after treatment with capsaicin. In control animals the density of immunoreactivity varied both with the transmitter and the type of neuron innervated. The density of immunoreactivity for all the three substances was reduced by capsaicin treatment. The degree of reduction of immunoreactivity in the fibers varied with the transmitter and the type of neuron. The density of CGRP and SP immunoreactive fibers was greatest around unlabeled neurons; 78% of the CGRP fibers were of primary sensory origin and all of the SP fibers were primary sensory. Around arterial neurons 44% of the CGRP fibers were of primary sensory origin and around venous 68% were primary sensory. NOS positive innervation around venous neurons was denser than around arterial neurons and all of it was completely (97%) eliminated by capsaicin, indicating that it was solely of primary sensory origin. We conclude that the primary sensory fibers innervating the IMG are differentially distributed to arterial and venous neurons and that the pattern of distribution is characteristic for each sensory neurotransmitter.
本研究的目的是确定豚鼠肠系膜下神经节（IMG）中，支配已鉴定血管神经元的初级感觉神经支配是否存在特定的组织形式。在体外将逆行示踪剂腔内注入肠系膜下动脉（IMA）或肠系膜下静脉（IMV），以将神经节神经元鉴定为动脉神经元、静脉神经元或未标记神经元。比较辣椒素处理前后，含有降钙素基因相关肽（CGRP）、神经元型一氧化氮合酶（NOS）和P物质免疫反应性（SP-IR）的初级感觉神经纤维的分布情况。在对照动物中，免疫反应性的密度随递质和所支配神经元的类型而变化。辣椒素处理后，所有这三种物质的免疫反应性密度均降低。纤维中免疫反应性的降低程度随递质和神经元类型而变化。CGRP和SP免疫反应性纤维的密度在未标记神经元周围最高；78%的CGRP纤维起源于初级感觉神经，所有SP纤维均为初级感觉神经。在动脉神经元周围，44%的CGRP纤维起源于初级感觉神经，在静脉神经元周围，68%起源于初级感觉神经。静脉神经元周围的NOS阳性神经支配比动脉神经元周围更密集，并且所有NOS阳性神经支配（97%）都被辣椒素完全消除，这表明其完全起源于初级感觉神经。我们得出结论，支配IMG的初级感觉纤维以不同方式分布于动脉和静脉神经元，并且每种感觉神经递质的分布模式都具有特征性。
