Heym C, Braun B, Klimaschewski L, Kummer W
Institute for Anatomy and Cell Biology, Ruprecht Karls University, Heidelberg, Germany.
Cell Tissue Res. 1995 Jan;279(1):169-81. doi: 10.1007/BF00300702.
Retrograde neuronal tracing in combination with double-labelling immunofluorescence was applied to distinguish the chemical coding of guinea-pig primary sensory neurons projecting to the adrenal medulla and cortex. Seven subpopulations of retrogradely traced neurons were identified in thoracic spinal ganglia T1-L1. Five subpopulations contained immunolabelling either for calcitonin gene-related peptide (CGRP) alone (I), or for CGRP, together with substance P (II), substance P/dynorphin (III), substance P/cholecystokinin (IV), and substance P/nitric oxide synthase (V), respectively. Two additional subpopulations of retrogradely traced neurons were distinct from these groups: neurofilament-immunoreactive neurons (VI), and cell bodies that were nonreactive to either of the antisera applied (VII). Nerve fibers in the adrenal medulla and cortex were equipped with the mediator combinations I, II, IV and VI. An additional meshwork of fibres solely labelled for nitric oxide synthase was visible in the medulla. Medullary as well as cortical fibres along endocrine tissue apparently lacked the chemical code V, while in the external cortex some fibre exhibited code III. Some intramedullary neuronal cell bodies revealed immunostaining for nitric oxide synthase, CGRP or substance P, providing an additional intrinsic adrenal innervation. Perikarya, immunolabelled for nitric oxide synthase, however, were too few to match with the large number of intramedullary nitric oxide synthase-immunoreactive fibres. A non-sensory participation is also supposed for the particularly dense intramedullary network of solely neurofilament-immunoreactive nerve fibres. The findings give evidence for a differential sensory innervation of the guinea-pig adrenal cortex and medulla. Specific sensory neuron subpopulations suggest that nervous control of adrenal functions is more complex than hitherto believed.
采用逆行神经元追踪结合双标免疫荧光技术，以区分投射至豚鼠肾上腺髓质和皮质的初级感觉神经元的化学编码。在胸段脊髓神经节T1-L1中鉴定出7个逆行追踪神经元亚群。其中5个亚群的神经元分别仅对降钙素基因相关肽（CGRP）呈免疫标记（I），或对CGRP与P物质（II）、P物质/强啡肽（III）、P物质/胆囊收缩素（IV）、P物质/一氧化氮合酶（V）呈免疫标记。另外2个逆行追踪神经元亚群与这些亚群不同：神经丝免疫反应性神经元（VI），以及对所应用的任何一种抗血清均无反应的细胞体（VII）。肾上腺髓质和皮质中的神经纤维具有介质组合I、II、IV和VI。在髓质中可见一个仅标记一氧化氮合酶的额外纤维网络。沿内分泌组织的髓质和皮质纤维显然缺乏化学编码V，而在皮质外层一些纤维呈现编码III。一些髓内神经元细胞体显示出一氧化氮合酶、CGRP或P物质的免疫染色，提示存在额外的肾上腺内在神经支配。然而，标记一氧化氮合酶的胞体数量过少，无法与大量髓内一氧化氮合酶免疫反应性纤维相匹配。对于特别密集的仅神经丝免疫反应性神经纤维组成的髓内网络，也推测存在非感觉性参与。这些发现为豚鼠肾上腺皮质和髓质的差异性感觉神经支配提供了证据。特定的感觉神经元亚群表明，肾上腺功能的神经控制比迄今所认为的更为复杂。
