肾功能损害患者肾脏中甲状旁腺激素/甲状旁腺激素相关蛋白受体的下调。

Down-regulation of PTH/PTHrP receptor in the kidney of patients with renal impairment.

作者信息

Chen J, Zhang J, Lin S

机构信息

Department of Nephrology, Huashan Hospital, Shanghai Medical University, China.

出版信息

Chin Med J (Engl). 1998 Jan;111(1):24-7.

PMID:10322647

Abstract

OBJECTIVE

To investigate whether the down-regulation of renal parathyroid hormone/parathyroid hormone related protein (PTH/PTHrP) receptor messenger ribonucleic acid (mRNA) expression is a general phenomenon in patients with different stages of renal disease, besides chronic renal failure.

METHODS

Twenty-five patients were divided into the following groups: (1) chronic glomerulonephritis with normal renal function (GNN) (2) chronic glomerulonephritis with moderate renal insufficiency (GNI) (3) severe chronic renal failure undergoing maintenance dialysis (CRF) (4) acute renal failure during oliguric phase (ARF) (5) normal control without renal suffering (NC). Using relatively quantitative reverse transcription/polymerase chain reaction (RT/PCR) method, we investigated PTH/PTHrP receptor mRNA expression in renal specimens obtained through biopsy or operation. The levels of plasma C-terminal PTH, serum phosphorus and calcium were also observed at the same time.

RESULTS

Plasma C-terminal PTH levels in GNI, CRF and ARF patients were 1.90, 9.73 and 8.63 times higher than those in NC. However, the difference between GNN and NC was insignificant. CRF and ARF patients also presented obviously elevated serum phosphorus (1.61, 1.86 vs 1.14 mmol/L) and reduced serum calcium (1.82, 1.71 vs 2.26 mmol/L) compared with that in the control. These two parameters for GNN and GNI patients were normal. The levels of PTH/PTHrP receptor mRNA (corrected by beta-actin mRNA) in the kidney of GNN, GNI, CRF and ARF patients was markedly decreased by up to 35.7%, 68.5%, 77.9% and 92.2%, respectively.

CONCLUSIONS

The down-regulation of renal PTH/PTHrP receptor mRNA occurs much earlier than the changes of renal function, plasma PTH, serum phosphorus and calcium in the course of human renal disease.

摘要

目的

研究除慢性肾衰竭外，肾甲状旁腺激素/甲状旁腺激素相关蛋白（PTH/PTHrP）受体信使核糖核酸（mRNA）表达下调在不同阶段肾病患者中是否为普遍现象。

方法

25例患者分为以下几组：（1）肾功能正常的慢性肾小球肾炎（GNN）；（2）中度肾功能不全的慢性肾小球肾炎（GNI）；（3）接受维持性透析的严重慢性肾衰竭（CRF）；（4）少尿期急性肾衰竭（ARF）；（5）无肾脏疾病的正常对照（NC）。采用相对定量逆转录/聚合酶链反应（RT/PCR）方法，研究通过活检或手术获取的肾标本中PTH/PTHrP受体mRNA的表达。同时观察血浆C末端PTH、血清磷和钙水平。

结果

GNI、CRF和ARF患者的血浆C末端PTH水平分别是NC患者的1.90倍、9.73倍和8.63倍。然而，GNN和NC之间的差异不显著。与对照组相比，CRF和ARF患者的血清磷也明显升高（分别为1.61、1.86对1.14 mmol/L），血清钙降低（分别为1.82、1.71对2.26 mmol/L）。GNN和GNI患者的这两个参数正常。GNN、GNI、CRF和ARF患者肾脏中PTH/PTHrP受体mRNA（经β-肌动蛋白mRNA校正）水平分别显著降低高达35.7%、68.5%、77.9%和92.2%。