Braun Katja, Atmanspacher Felix, Schreckenberg Rolf, Grgic Ivica, Schlüter Klaus-Dieter
Physiologisches Institut, Justus-Liebig-Universität Gießen, Gießen, Germany.
Klinik für Innere Medizin und Nephrologie, Philipps-Universität Marburg, Marburg, Germany.
Physiol Rep. 2018 Sep;6(17):e13842. doi: 10.14814/phy2.13842.
An active lifestyle is generally recommended for hypertensive patients to prevent subsequent end-organ damage. However, experimental data on long-term effects of exercise on hypertension are insufficient and underlying mechanisms are not well understood. This study was aimed to investigate the effect of exercise on renal expression of parathyroid hormone-related protein (PTHrP) and parathyroid hormone receptor type 1 (PTHR1) in spontaneously hypertensive rats (SHR). Twenty-four rats started free running wheel exercise at the age of 1.5 months (pre-hypertensive state) and proceeded for 1.5, 3.0, 6.0, and 10.0 months. Thirty rats kept under standard housing conditions were used as sedentary controls. Kidney function was assessed by measuring plasma creatinine levels and urine albumin-to-creatinine ratios. Renal expression of PTHrP and PTHR1 was analyzed by qRT-PCR and western blot. Renal expression of PTHR1 was markedly increased between the 6th and 10th months in sedentary rats and this increase was significantly lower in SHRs with high physical activity on mRNA (-30%) and protein level (-27%). At the same time, urine albumin-to-creatinine ratio increased (from 65 to 231 mg/g) but somehow lower in exercise performing SHRs (48-196 mg/g). Our data suggest that enhanced exercise, stimulated by allocation of a free running wheel, is associated with lower PTHR1 expression in SHRs and this may contribute to preserved kidney function.
一般建议高血压患者保持积极的生活方式，以预防随后的靶器官损害。然而，关于运动对高血压长期影响的实验数据不足，其潜在机制也尚未完全了解。本研究旨在探讨运动对自发性高血压大鼠（SHR）肾脏中甲状旁腺激素相关蛋白（PTHrP）和1型甲状旁腺激素受体（PTHR1）表达的影响。24只大鼠在1.5月龄（高血压前期）开始自由轮转运动，并持续1.5、3.0、6.0和10.0个月。30只饲养在标准条件下的大鼠作为久坐对照组。通过测量血浆肌酐水平和尿白蛋白与肌酐比值来评估肾功能。采用qRT-PCR和western blot分析PTHrP和PTHR1的肾脏表达。在久坐大鼠中，PTHR1的肾脏表达在第6个月至第10个月显著增加，而在高运动量的SHR中，这种增加在mRNA水平（-30%）和蛋白质水平（-27%）上显著降低。同时，尿白蛋白与肌酐比值升高（从65至231mg/g），但在进行运动的SHR中该比值有所降低（48-196mg/g）。我们的数据表明，自由轮转运动刺激的增强运动与SHR中较低的PTHR1表达相关，这可能有助于维持肾脏功能。
