Gui S Q, Yu J, Wei M J
Obstetrics and Gynecology Hospital, Shanghai Medical University.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 1997 Dec;17(12):735-8.
To observe the effect of tonifying Kidney herbs (TKH) in pituitary, ovary, adrenal gland of androgen sterilized rats(ASR).
ASR model was established by injecting testosterone propionate subcutaneously to SD female rats of 9 days age. Morphological and hormonal change of pituitary, ovary and adrenal gland in rats of 100-107 days old before and after feeding TKH extract were observed by light and electron microscope, cell culture immunohistochemical studies and radioimmuno-assay.
In the ASR groups, there were intracytopiasmic lipid drops, autophagy, vacuole, granulolysis of pituitary gland. There were anovulation apparently and increased the amount of lipid drops in cytoplasm of interstitial glandular cell of ovary. The fatty drops of the reticular zone of adrenal gland decreased, the numbers of AGNOR (P < 0.01) and PCNA (P < 0.01) increased significantly. The levels of FSH, LH (P < 0.05-0.005) lowered, but the levels of DHA, T (P < 0.01, 0.05, 0.001) were raised significantly. Morphological and hormonal change of all RSA returned to normal range after the treatment.
The hyperandrogenemia were disturbed by the function of sex gland axis and adrenal in female rats of 9 days old and played an important role in pathogenesis of ASR, the TKH reduced the levels of androgen and induced ovulation through sex gland axis and adrenal level and multi-organ regulation.
观察补肾中药对雄激素致不孕大鼠垂体、卵巢、肾上腺的影响。
将丙酸睾酮皮下注射于9日龄SD雌性大鼠建立雄激素致不孕大鼠模型。采用光镜、电镜、细胞培养免疫组化及放射免疫分析法，观察100 - 107日龄大鼠喂饲补肾中药提取物前后垂体、卵巢和肾上腺的形态及激素变化。
雄激素致不孕大鼠组垂体细胞内有脂滴、自噬、空泡形成及颗粒溶解；卵巢明显无排卵，间质腺细胞胞质内脂滴增多；肾上腺网状带脂滴减少，核仁组成区嗜银蛋白（P < 0.01）和增殖细胞核抗原（P < 0.01）数量显著增加；促卵泡生成素、促黄体生成素水平降低（P < 0.05 - 0.005），但脱氢表雄酮、睾酮水平显著升高（P < 0.01、0.05、0.001）。治疗后，所有雄激素致不孕大鼠的形态及激素变化均恢复至正常范围。
9日龄雌性大鼠性腺轴和肾上腺功能紊乱导致高雄激素血症，在雄激素致不孕发病机制中起重要作用，补肾中药通过性腺轴和肾上腺水平及多器官调节降低雄激素水平并诱导排卵。
