Xu Ding-Jie, Hong Li-Wen, Xu Hong, Yang Hong-Min, Liu Man-Fang, Du Hui-Lan
College of Traditional Chinese Medicine, Hebei United University, Hebei 063000, China.
Department of Integrative Medical Gynecology, Heibei Medical University, Shijiazhuang 050091, China.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2014 Jan;34(1):87-90.
To observe the effect of Bushen Tiaojing Recipe (BTR) and Xiaoyao Pill (XYP) on the morphology and sex hormones secretion of adenohypophysis and ovaries in androgen-induced sterile rats (ASR).
Fifty 9-day old SD female rats randomly recruited from total 60 rats were subcutaneously injected with testosterone propionate to establish the ASR model. And the rest 10 rats were recruited as the normal group. Thirty successfully modeled rats were recruited and randomly divided into the model group, the BTR group (administered with BTR suspension), and the XYP group (administered with XYP suspension), 10 in each group. Five weeks later, rats were decapitated in the proestrus. Serum levels of estradiol (E2), progesterone (P), testosterone (T), follicle-stimulating hormone (FSH), and luteinizing hormone (LH) were detected by radioimmunoassay. The morphologies of adenohypophysis and ovary were observed after HE staining.
Compared with the normal group, serum E2 and T levels increased, while FSH and LH levels decreased in the model group (all P < 0.01). The morphology of adenohypophysis and ovary was abnormal in the model group. Compared with the model group, serum E2 and T levels decreased, while FSH and LH levels increased in the BTR group and the XYP group (P < 0.05, P < 0.01). Besides, E2 and T levels in the BTR group and FSH levels in the XYP group restored to normal (all P > 0.05). The damaged structure of adenohypophysis and ovary got restored to different degrees.
BTR and XYP both could improve ovulation failure.
观察补肾调经方（BTR）与逍遥丸（XYP）对雄激素致不孕大鼠（ASR）腺垂体及卵巢形态和性激素分泌的影响。
从60只大鼠中随机选取50只9日龄SD雌性大鼠，皮下注射丙酸睾酮建立ASR模型。其余10只大鼠作为正常组。将30只成功造模的大鼠随机分为模型组、补肾调经方组（给予补肾调经方混悬液）和逍遥丸组（给予逍遥丸混悬液），每组10只。5周后，在动情前期将大鼠断头。采用放射免疫法检测血清雌二醇（E2）、孕酮（P）、睾酮（T）、促卵泡生成素（FSH）和促黄体生成素（LH）水平。HE染色后观察腺垂体和卵巢的形态。
与正常组相比，模型组血清E2和T水平升高，而FSH和LH水平降低（均P<0.01）。模型组腺垂体和卵巢形态异常。与模型组相比，补肾调经方组和逍遥丸组血清E2和T水平降低，而FSH和LH水平升高（P<0.05，P<0.01）。此外，补肾调经方组E2和T水平以及逍遥丸组FSH水平恢复正常（均P>0.05）。腺垂体和卵巢受损结构不同程度恢复。
补肾调经方和逍遥丸均能改善排卵障碍。
