Lei Y, Shi D Z, Zhong P
Xiyuan Hospital, China Academy of TCM, Beijing.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 1997 Oct;17(10):607-10.
To explore the effect of Yuxintong (YXT) on the endothelin converting enzyme (ECE) and the nitric oxide synthase (NOS) gene expression levels and cooper-zinc-superoxide dismutase (SOD) activity.
The acute myocardial ischemia models were reproduced in anesthetized dogs by ligating the left coronary artery at the middle of anterior descending branch. The ECE and NOS gene expressions were observed by in situ hybridization and the cooper-zinc-SOD was studied by immunohistochemical and image analysis.
It was found that the transfer of ECE mRNA was greatly increased, while that of NOS mRNA was greatly decreased in ischemic my ocardium, but the YXT could enhance the expression levels of NOS mRNA and inhibit the over expression of ECE mRNA in ischemic myocardium in dog. Meanwhile, the cooper-zinc-SOD immunohistochemistry was significantly weakened in ischemic myocardium, but the YXT could evidently increase SOD activity in experimental ischemic myocardium.
It suggested that YXT had notable protection on experimental ischemic myocardium and was definitely valuable on prevention and treatment of coronary heart disease.
探讨愈心痛（YXT）对内皮素转换酶（ECE）、一氧化氮合酶（NOS）基因表达水平及铜锌超氧化物歧化酶（SOD）活性的影响。
通过结扎麻醉犬左冠状动脉前降支中段复制急性心肌缺血模型。采用原位杂交观察ECE和NOS基因表达，采用免疫组织化学及图像分析研究铜锌超氧化物歧化酶。
发现缺血心肌中ECE mRNA表达显著增加，而NOS mRNA表达显著降低，但YXT可提高犬缺血心肌中NOS mRNA表达水平，抑制ECE mRNA的过度表达。同时，缺血心肌中铜锌超氧化物歧化酶免疫组织化学明显减弱，但YXT可明显增加实验性缺血心肌中的SOD活性。
提示YXT对实验性缺血心肌有显著保护作用，对冠心病的防治具有一定价值。
