2.5s小鼠神经生长因子对小鼠和大鼠坐骨神经损伤后再生的影响。

Effects of 2.5 s mouse nerve growth factor on regeneration of injured sciatic nerves in mice and rats.

作者信息

Wang J Z, Bai D C, Rao C M, Li W G, Gao M T, Wang X Q, Wu Y J

机构信息

National Institute for the Control of Pharmaceutical and Biological Products, Beijing, China.

出版信息

Zhongguo Yao Li Xue Bao. 1997 Nov;18(6):501-4.

PMID:10322904

Abstract

AIM

To study the promoting activity of 2.5 s murine nerve growth factor (NGF) for the regeneration of injuried sciatic nerve.

METHODS

The sciatic nerves of mice and rats were crushed. The axon counts and differential axon counts were made. Evoked potential latency (NMEPL) was recorded at sites of proximal (Pro) and distal (Dis) segment of soleus muscles.

RESULTS

In mice, NGF im 0.5-1 kBU.kg-1 increased the axon counts of regenerated nerves 20 d after crush; 2-4 kBU.kg-1 simultaneously alleviated the denervated atrophy of soleus muscles. In rats, NGF i.m. increased the axon counts at dose of 1 kBU.kg-1 for 40 d, 2 kBU.kg-1 for 30 d and 40 d, and 4 kBU.kg-1 for 20 d, 30 d and 40 d after crush; the number of large axons of regenerated nerves were also increased by NGF i.m. 2 kBU.kg-1 for 40 d and 4 kBU.kg-1 for 30 d and 40 d; Pro NMEPL was shortened by NGF 1, 2, and 4 kBU.kg-1, Dis-NMEPL was shortened by NGF 2 and 4 kBU.kg-1 for 40 d.

CONCLUSION

NGF im evidently promotes the regeneration of injuried sciatic nerve, and attenuates the skeletal muscular atrophy caused by nerve injury.

摘要

目的

研究2.5s鼠神经生长因子（NGF）对损伤坐骨神经再生的促进作用。

方法

对小鼠和大鼠的坐骨神经进行挤压。进行轴突计数和不同类型轴突计数。记录比目鱼肌近端（Pro）和远端（Dis）部位的诱发电位潜伏期（NMEPL）。

结果

在小鼠中，0.5 - 1kBU.kg-1剂量的NGF肌肉注射可增加挤压后20天再生神经的轴突计数；2 - 4kBU.kg-1剂量同时可减轻比目鱼肌的失神经萎缩。在大鼠中，1kBU.kg-1剂量的NGF肌肉注射在挤压后40天增加轴突计数，2kBU.kg-1剂量在挤压后30天和40天增加轴突计数，4kBU.kg-1剂量在挤压后20天、30天和40天增加轴突计数；2kBU.kg-1剂量肌肉注射40天以及4kBU.kg-1剂量肌肉注射30天和40天可增加再生神经的大轴突数量；1、2和4kBU.kg-1剂量的NGF可缩短Pro NMEPL，2和4kBU.kg-1剂量的NGF肌肉注射40天可缩短Dis-NMEPL。