Chen Z W, Wang M S
Department of Orthopaedics, Zhongshan Hospital, Shanghai Medical University, Shanghai, People's Republic of China.
Microsurgery. 1995;16(8):547-51. doi: 10.1002/micr.1920160808.
The effects of nerve growth factor (NGF) on crushed sciatic nerve regeneration were studied in 30 rats, with 60 bilateral nerves. The nerves were crushed at a site 6 mm distal to the sciatic notch by the standard technique and 3 mm wide crush injuries were created. Then 2.1 microliters of normal saline in the control groups and an equal volume of NGF solution (containing 1 microgram of NGF) in the NGF-treated groups was injected into the crush sites and followed for 12, 28, and 56 days, respectively. At the end of the observation, electrophysiological evaluation was carried out; then samples 10 mm distal to the crush site were removed and prepared for histological and morphometric studies. Evoked muscle action potential (MAP) was recorded in 50% of the NGF-treated group at 12 days but not in the control group; the difference was statistically significant (P < 0.05). The motor nerve conduction velocity (MNCV) was increased in NGF-treated groups compared with control groups at 28 and 56 days (P < 0.05). Morphometrically, significantly more regenerated myelinated fibers (RMFs) were seen at 12 days, and larger diameter RMFs were found at 12, 28, and 56 days in NGF-treated groups than in control groups. These results indicate that topically applied NGF stimulates nerve regeneration and promotes function recovery in crushed rat sciatic nerves.
在30只大鼠（共60条双侧坐骨神经）中研究了神经生长因子（NGF）对坐骨神经挤压伤后再生的影响。采用标准技术在坐骨切迹远端6 mm处挤压神经，造成3 mm宽的挤压伤。然后，对照组在挤压部位注射2.1微升生理盐水，NGF治疗组注射等体积的NGF溶液（含1微克NGF），分别随访12天、28天和56天。观察结束时进行电生理评估；然后在挤压部位远端10 mm处取材，准备进行组织学和形态学研究。在12天时，50%的NGF治疗组记录到诱发肌肉动作电位（MAP），而对照组未记录到；差异具有统计学意义（P<0.05）。在28天和56天时，NGF治疗组的运动神经传导速度（MNCV）高于对照组（P<0.05）。形态学上，在12天时，NGF治疗组可见明显更多的再生有髓纤维（RMF），且在12天、28天和56天时，NGF治疗组的RMF直径大于对照组。这些结果表明，局部应用NGF可刺激大鼠坐骨神经挤压伤后的神经再生并促进功能恢复。
