[颈髓损伤后线粒体酶活性改变与线粒体功能的关系]

[The relationship between alteration of mitochondrial enzyme activities and mitochondrial function after cervical cord injury].

作者信息

Xiao J, Fu Q, Hou T, Li Y, Zhao D

机构信息

ChangZheng Hospital, Second Military Medical University, Shanghai.

出版信息

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 1997 Nov;13(4):326-8.

PMID:10322962

Abstract

To investigate the relationship between mitochondrial enzyme activities and mitochondrial function after cervical cord injury, cervical cord model was prepared using Allen method in cats. Changes in mitochondrial Na+, K(+)-ATPase, Ca2+, Mg(2+)-ATPase, superoxide dismutase (SOD) activities, contents of malondialdehyde (MDA) and mitochondrial function in cervical cord were observed by different biochemical methods. The results showed that mitochondrial Na+, K(+)-ATPase, CA2+, Mg(2+)-ATPase, SOD activities decreased; mitichondral respiratory control rate (RCR), P/O ratio and oxidative phosphorylation rate (OPR) were also depressed is compared with control group (P < 0.05), but MDA level significantly increased compared to control group (P < 0.01). The results demonstrate that there is close correlation, between mitochondrial ATPase. SOD activities and mitochondral function, suggesting that mitochondrial pathophysiological alteration plays important roles in the secondary damage after cervical cord injury.

摘要

为研究颈髓损伤后线粒体酶活性与线粒体功能之间的关系，采用Allen法在猫身上制备颈髓损伤模型。通过不同的生化方法观察颈髓中线粒体Na +、K(+)-ATP酶、Ca2 +、Mg(2+)-ATP酶、超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量及线粒体功能的变化。结果显示，与对照组相比，线粒体Na +、K(+)-ATP酶、Ca2 +、Mg(2+)-ATP酶、SOD活性降低；线粒体呼吸控制率（RCR）、P/O比值和氧化磷酸化率（OPR）也降低（P < 0.05），但MDA水平较对照组显著升高（P < 0.01）。结果表明，线粒体ATP酶、SOD活性与线粒体功能之间存在密切相关性，提示线粒体病理生理改变在颈髓损伤后的继发性损伤中起重要作用。