Yanase T, Yasunaga C, Hara T, Kawarabata T
Institute of Biological Control, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Intervirology. 1998;41(6):244-52. doi: 10.1159/000024946.
The Autographa californica multinucleocapsid nuclear polyhedrosis virus (AcMNPV) has a broad host range among Lepidoptera. In contrast, the Spodoptera exigua MNPV (SeMNPV) can replicate efficiently only in S. exigua larvae or S. exigua-derived cell lines. In this study, we examined the coinfection of S. exigua Se301 and Spodoptera frugiperda IPLB-SF21AEII (Sf21) cell lines with SeMNPV and AcMNPV recombinant (Ac360-501beta-gal) which was constructed for expression of beta-galactosidase under control of the polyhedrin promoter. Coinfection led to the restriction as the level of late gene expression, nonoccluded virus production, and DNA replication of Ac360-501beta-gal in both Se301 and Sf21 cell lines. In contrast, Ac360-501beta-gal supported the SeMNPV replication in Sf21 cells. Occurrence of recombinants, between Ac360-501beta-gal and SeMNPV, with expanded host range was not observed in coinfected Sf21 cells. This suggests that Ac360-501beta-gal supports the SeMNPV replication through trans-activation.
苜蓿银纹夜蛾多核衣壳核型多角体病毒（AcMNPV）在鳞翅目昆虫中有广泛的宿主范围。相比之下，甜菜夜蛾核型多角体病毒（SeMNPV）只能在甜菜夜蛾幼虫或源自甜菜夜蛾的细胞系中高效复制。在本研究中，我们检测了甜菜夜蛾Se301细胞系和草地贪夜蛾IPLB-SF21AEII（Sf21）细胞系与SeMNPV和AcMNPV重组体（Ac360-501β-半乳糖苷酶）的共感染情况，该重组体构建用于在多角体蛋白启动子控制下表达β-半乳糖苷酶。共感染导致Ac360-501β-半乳糖苷酶在Se301和Sf21细胞系中的晚期基因表达水平、非包涵体病毒产生和DNA复制受到限制。相反，Ac360-501β-半乳糖苷酶在Sf21细胞中支持SeMNPV的复制。在共感染的Sf21细胞中未观察到Ac360-501β-半乳糖苷酶和SeMNPV之间出现宿主范围扩大的重组体。这表明Ac360-501β-半乳糖苷酶通过反式激活支持SeMNPV的复制。
