Yanase T, Yasunaga C, Kawarabata T
Institute of Biological Control, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Acta Virol. 1998 Nov;42(5):293-8.
The Spodoptera exigua multinucleocapsid nucleopolyhedrovirus (SeMNPV) was inoculated to eight lepidopteran cell lines derived from Spodoptera exigua (Se301), Spodoptera frugiperda (SF21AEII), Spodoptera littoralis (CLS-79), Spodoptera litura (SpLi-221), Pseudaletia separata (LeSe-11), Trichoplusia ni (hi-5), Plutella xylostella (PXL/C) and Bombyx mori (BmN4). The productive infection of SeMNPV was observed only in Se301 cells. However, a dot-blot hybridization analysis revealed that SeMNPV DNA replicated in five non-permissive cell lines: SF21AEII, CLS-79, SpLi-221, hi-5 and BmN4. In addition, the virus-infected hi-5 and BmN4 cells displayed morphological changes. In contrast, CLS-79 cells inoculated with SeMNPV showed membrane blebbing at 20 hrs post inoculation (p.i.) and fragmentation of genomic DNA. All that indicated that the infected CLS-79 cells underwent apoptosis. These findings indicate that the SeMNPV replication was restricted at various points in dependence upon each cell line.
将甜菜夜蛾多核衣壳核型多角体病毒（SeMNPV）接种到8种鳞翅目细胞系中，这些细胞系分别来源于甜菜夜蛾（Se301）、草地贪夜蛾（SF21AEII）、埃及棉铃虫（CLS - 79）、斜纹夜蛾（SpLi - 221）、粘虫（LeSe - 11）、粉纹夜蛾（hi - 5）、小菜蛾（PXL/C）和家蚕（BmN4）。仅在Se301细胞中观察到SeMNPV的有效感染。然而，斑点杂交分析显示SeMNPV DNA在5种非允许细胞系中复制：SF21AEII、CLS - 79、SpLi - 221、hi - 5和BmN4。此外，病毒感染的hi - 5和BmN4细胞出现了形态变化。相比之下，接种SeMNPV的CLS - 79细胞在接种后20小时（p.i.）出现膜泡化和基因组DNA片段化。所有这些表明受感染的CLS - 79细胞发生了凋亡。这些发现表明SeMNPV的复制在不同细胞系中受到不同程度的限制。
