Tan M, Wang L E, Xie D, Li Y
Institute of Occupational Medicine, Chinese Academy of Preventive Medicine, Beijing, China.
Wei Sheng Yan Jiu. 1997 May;26(3):159-61.
The human embryonic lung fibroblasts transformation was induced by chemical mutant Glycidyl Methacrylate (GMA) in vitro. Transformed clonies were isolated and then exon 5 and exon 8 of p53 gene were specifically amplified by polymerase chain reaction. Amplified fragments were detected by using silver stained single strand conformation polymorphism analysis. The results showed that exon 8 altered in the transformed cells, indicating that p53 gene played an important role in the human embryonic lung fibroblasts transformation induced by GMA.
在体外，化学诱变剂甲基丙烯酸缩水甘油酯（GMA）诱导人胚肺成纤维细胞发生转化。分离出转化克隆，然后通过聚合酶链反应特异性扩增p53基因的第5外显子和第8外显子。采用银染单链构象多态性分析检测扩增片段。结果显示，转化细胞中第8外显子发生改变，表明p53基因在GMA诱导的人胚肺成纤维细胞转化中起重要作用。
