Xu J, Yin X, Wang Q, Li Z
Institute of Occupational Medicine, Chinese Academy of Preventive Medicine, Beijing 100050, China.
Wei Sheng Yan Jiu. 2001 Jan;30(1):14-7.
The malignant transformation of human embryonic lung fibroblasts (HELF) induced by glycidyl methacrylate (GMA) in vitro was used as a model for comparing gene expression between the transformed cells and controls. Using mRNA differential display PCR(DD-PCR) technique, the three cDNA fragments up-regulated in the transformed cells were identified and highly homologous to human breast cancer transcription factor(ZABC1), Tbx3 and mouse ubiquitin conjugating enzyme(E2) gene. These results suggest that activation or inactivation of genes including ZABC1, Tbx3 and E2 may be involved in GMA-induced transformation.
以甲基丙烯酸缩水甘油酯（GMA）体外诱导人胚肺成纤维细胞（HELF）恶性转化作为模型，比较转化细胞与对照细胞之间的基因表达。采用mRNA差异显示PCR（DD-PCR）技术，鉴定出转化细胞中上调的三个cDNA片段，它们与人乳腺癌转录因子（ZABC1）、Tbx3和小鼠泛素结合酶（E2）基因高度同源。这些结果表明，包括ZABC1、Tbx3和E2在内的基因的激活或失活可能参与了GMA诱导的转化过程。
