Yamaguchi K, Murakami M, Takahashi K, Moriya H, Tatsuoka H, Chiba T
Department of Orthopaedic Surgery, School of Medicine, Chiba University, Japan.
Spine (Phila Pa 1976). 1999 May 1;24(9):845-51. doi: 10.1097/00007632-199905010-00003.
An experimental model in rats of chronically compressed cauda equina was produced, and behavioral and morphologic changes were examined.
To provide a useful model for analyzing the pathophysiologic changes of the cauda equina by chronic compression and to examine behavioral and morphologic changes in this model.
Several animal models have been reported in which various materials were used to compress the cauda equina. However, the pathophysiology of the cauda equina by chronic compression is not yet well understood. Studies in which rats were used are scarce.
A silicone sheet was applied to the spinal canal at L4 in the rat. Walking durations on treadmill tests and paw-withdrawal latencies to thermal stimuli were measured before and after the operation for 24 weeks. Histologic changes also were examined.
Walking durations decreased after chronic compression. However, paw-withdrawal latencies were not significantly changed. Histologically, the number of large-diameter myelinated axons decreased after compression, whereas the number of small-diameter myelinated axons increased. Electron microscopic observation indicated that the continuous degeneration and regeneration of axons occurred throughout the chronic compression experiment.
The current model and behavioral assessments may be useful in analyzing the pathophysiology of chronically compressed cauda equina.
建立大鼠慢性压迫马尾神经的实验模型，并检测行为和形态学变化。
为分析慢性压迫导致的马尾神经病理生理变化提供有用模型，并检测该模型中的行为和形态学变化。
已有多种动物模型报道，其中使用了各种材料来压迫马尾神经。然而，慢性压迫导致的马尾神经病理生理学尚未得到充分理解。使用大鼠的研究很少。
将硅橡胶片应用于大鼠L4水平的椎管。在手术前后24周测量跑步机试验中的行走时间和对热刺激的爪部退缩潜伏期。还检查了组织学变化。
慢性压迫后行走时间减少。然而，爪部退缩潜伏期没有显著变化。组织学上，压迫后大直径有髓轴突数量减少，而小直径有髓轴突数量增加。电子显微镜观察表明，在整个慢性压迫实验过程中轴突持续发生退变和再生。
当前模型和行为评估可能有助于分析慢性压迫马尾神经的病理生理学。
