Shofuda K, Togawa T, Nakato H, Tomino S, Izumi S
Department of Biology, Tokyo Metropolitan University, Minamiosawa, Japan.
Comp Biochem Physiol B Biochem Mol Biol. 1999 Jan;122(1):105-9. doi: 10.1016/s0305-0491(98)10151-7.
Cuticle proteins termed LCPs are the major protein components of the larval integument of the silkworm, Bombyx mori. We purified an 18 kDa LCP (LCP18) from the guanidine hydrochloride extract of the larval cuticle and identified an LCP18 cDNA clone. The deduced primary structure and mRNA expression pattern of LCP18 are similar to those of other Bombyx LCPs and to several cuticle proteins identified in other insect species. RNA blot analysis demonstrated that the biosynthesis of LCP18 is regulated in a stage-dependent manner at the level of mRNA in epidermal cells. An in vivo study using a juvenile hormone analogue suggested that juvenile hormone positively regulates expression of LCP18 mRNA during larval intermolt stages.
被称为LCPs的表皮蛋白是家蚕幼虫体表的主要蛋白质成分。我们从幼虫表皮的盐酸胍提取物中纯化出一种18 kDa的LCP（LCP18），并鉴定出一个LCP18 cDNA克隆。LCP18推导的一级结构和mRNA表达模式与其他家蚕LCPs以及在其他昆虫物种中鉴定出的几种表皮蛋白相似。RNA印迹分析表明，LCP18的生物合成在表皮细胞中mRNA水平上受到阶段依赖性调控。一项使用保幼激素类似物的体内研究表明，保幼激素在幼虫龄期之间的阶段对LCP18 mRNA的表达起正向调节作用。
