Kytölä J, Uotila P, Kääpä P
Department of Pediatrics, University of Turku, Finland.
Prostaglandins Leukot Essent Fatty Acids. 1999 Feb;60(2):107-10. doi: 10.1054/plef.1998.0015.
Since meconium aspiration often induces an inflammatory respiratory disorder, we investigated the effects of intrapulmonary meconium on the expression of cyclooxygenase-1 and 2 in rat lungs. Suspension of human meconium was instilled intratracheally into ventilated lungs of anesthetized rats, while control rats received an equal volume of saline. The meconium lungs were ventilated either with air or 100% oxygen, and control lungs were ventilated with air. After 3 h, the lungs were removed and the amount of cyclooxygenase-1 and 2 mRNA was measured by Northern blot analysis. Cyclooxygenase-1 mRNA was clearly expressed in control rat lungs, while cyclooxygenase-2 expression was minimal. Meconium administration markedly upregulated the expression of cyclooxygenase-2 mRNA, while cyclooxygenase-1 expression remained unchanged. Increased expression of cyclooxygenase-2 was detected in rat lungs ventilated with either air or oxygen. Our data thus indicate that meconium aspiration induces pulmonary expression of cyclooxygenase-2, suggesting an important role for prostaglandins in the meconium aspiration-induced inflammation in neonatal lungs.
由于胎粪吸入常引发炎症性呼吸系统疾病，我们研究了肺内胎粪对大鼠肺中环氧合酶-1和环氧合酶-2表达的影响。将人胎粪悬液经气管内滴入麻醉大鼠的通气肺中，而对照大鼠接受等量的生理盐水。胎粪处理的肺用空气或100%氧气通气，对照肺用空气通气。3小时后，取出肺脏，通过Northern印迹分析测量环氧合酶-1和环氧合酶-2 mRNA的量。环氧合酶-1 mRNA在对照大鼠肺中清晰表达，而环氧合酶-2的表达极少。给予胎粪显著上调了环氧合酶-2 mRNA的表达，而环氧合酶-1的表达保持不变。在用空气或氧气通气的大鼠肺中均检测到环氧合酶-2表达增加。因此，我们的数据表明胎粪吸入诱导肺中环氧合酶-2的表达，提示前列腺素在新生儿肺胎粪吸入诱导的炎症中起重要作用。
