Gerbens F, van Erp A J, Harders F L, Verburg F J, Meuwissen T H, Veerkamp J H, te Pas M F
Department of Breeding and Genetics, DLO-Institute for Animal Science and Health (ID-DLO), Lelystad, The Netherlands.
J Anim Sci. 1999 Apr;77(4):846-52. doi: 10.2527/1999.774846x.
In order to find genetic markers to improve the meat quality of pigs by breeding we studied the relationship between variation in the heart fatty acid-binding protein (H-FABP) gene (FABP3) and intramuscular fat (IMF) content. To estimate the effect of H-FABP, pigs from two Duroc populations were selectively mated in such a way that at least two genotypes were present in each litter. In total, data from 983 pigs and pedigree information from three preceding generations were analyzed. Offspring were tested for IMF content as well as backfat thickness (BFT), BW, and drip loss of the meat (DRIP). All pigs were assigned to H-FABP RFLP genotype classes either by the assessed genotype (75%) or based on a probability score determined according to genotypic information of their relatives (25%). Contrasts were detected between homozygous H-FABP RFLP genotype classes for IMF content (.4%, P < .05), BFT (.6 mm, P < .01), and BW (2.4 kg, P < .10). No significant contrasts were detected for DRIP. Results for IMF content, BFT, and BW were confirmed when only genotyped animals were analyzed. Variation in BFT partially explained the effect on IMF content. Although other closely linked genes on porcine chromosome 6 might be responsible for the observed effect, interference of the halothane gene was excluded because all parental animals were noncarriers. In conclusion, H-FABP RFLP can be used as markers to select for increased IMF content and growth in breeding programs.
为了通过育种找到改善猪肉品质的遗传标记，我们研究了心脏脂肪酸结合蛋白（H-FABP）基因（FABP3）变异与肌内脂肪（IMF）含量之间的关系。为评估H-FABP的作用，对两个杜洛克猪群体进行选择性配种，使每窝至少有两种基因型。总共分析了983头猪的数据以及前三代的系谱信息。对后代进行了IMF含量、背膘厚度（BFT）、体重（BW）和肉的滴水损失（DRIP）检测。所有猪根据评估的基因型（75%）或根据其亲属的基因型信息确定的概率得分（25%）被分配到H-FABP RFLP基因型类别。在纯合H-FABP RFLP基因型类别之间检测到IMF含量（.4%，P <.05）、BFT（.6 mm，P <.01）和BW（2.4 kg，P <.10）的差异。未检测到DRIP的显著差异。仅分析基因分型动物时，IMF含量、BFT和BW的结果得到证实。BFT的变异部分解释了对IMF含量的影响。尽管猪6号染色体上其他紧密连锁的基因可能是观察到的效应的原因，但由于所有亲本动物均为非携带者，排除了氟烷基因的干扰。总之，H-FABP RFLP可作为标记，在育种计划中用于选择增加IMF含量和生长性能。
