Vercelli A, Repici M, Biasiol S, Jhaveri S
Department of Anatomy, Pharmacology and Forensic Medicine, University of Torino, Turin, 10126, Italy.
Exp Neurol. 1999 Apr;156(2):294-315. doi: 10.1006/exnr.1999.7025.
Histochemical detection of NADPH-d activity in rat barrel-field cortex reveals four types of distributions. (i) A transient, diffuse neuropil staining is visible in the cortical plate and in deeper layers until postnatal day (P) 4. Thereafter, until P15, it is segregated in whisker-specific patches in layer IV, then the pattern gradually disappears, becoming virtually indistinct by P21. This transient patterning of diffuse NADPH-d activity in layer IV disappears after cortical injections of kainic acid and is affected by neonatal damage to the contralateral snout. An intense labeling (ii) of scattered cells and (iii) of a plexus of fibers is present. With maturation, the cells become localized mostly in layers II/III, in the lower part of layer V, and in layer VI. They are sparse in layer I, in upper layer V, and in layer IV where their somata are located primarily in the interbarrel septa. (iv) Light staining of cortical neurons is detected mostly in layers II-IV but occasionally also in layers V-VI. Cytochrome c oxidase (CO)-positive patches associated with barrels are first detected in layer IV around P4-P5; their staining density increases with development, then stays high. In the adult, CO activity is moderate in supragranular layers, highest in the barrels in layer IV, low in upper layer V, medium dense in the deeper half of layer V, and low in lamina VI. Thus, NADPH-d and CO activities are not necessarily colocalized in the rodent barrel-field cortex. The varied (transient and long-lasting) distributions of NADPH-d activity indicate that the enzyme and its associated production of NO serve multiple roles in developing and adult barrel-field cortex.
大鼠桶状皮质中NADPH-d活性的组织化学检测揭示了四种分布类型。(i) 在出生后第4天之前，皮质板和更深层可见短暂的、弥漫性的神经毡染色。此后，直到出生后第15天，它在第IV层中分隔成特定触须的斑块，然后这种模式逐渐消失，到出生后第21天几乎变得不明显。在皮质注射谷氨酸后，第IV层中弥漫性NADPH-d活性的这种短暂模式消失，并且受到对侧口鼻部新生损伤的影响。(ii) 存在散在细胞的强烈标记和(iii) 纤维丛的强烈标记。随着成熟，这些细胞主要定位于第II/III层、第V层下部和第VI层。它们在第I层、第V层上部和第IV层中稀疏，其胞体主要位于桶间隔中。(iv) 皮质神经元的浅染色主要在第II-IV层中检测到，但偶尔也在第V-VI层中检测到。与桶相关的细胞色素c氧化酶(CO)阳性斑块在出生后第4 - 5天左右在第IV层中首次检测到；它们的染色密度随着发育增加，然后保持高水平。在成体中，CO活性在颗粒上层中中等，在第IV层的桶中最高，在第V层上部低，在第V层下半部中等密度，在第VI层低。因此，NADPH-d和CO活性在啮齿动物桶状皮质中不一定共定位。NADPH-d活性的多样(短暂和持久)分布表明该酶及其相关的NO产生在发育中和成年桶状皮质中发挥多种作用。
