Zheng Y L, Zhen D K, Farina A, Berry S M, Wapner R J, Williams J M, Bianchi D W
Departments of Pediatrics, New England Medical Center Hospital/Tufts, Boston, MA 02111, USA.
Am J Obstet Gynecol. 1999 May;180(5):1234-9. doi: 10.1016/s0002-9378(99)70622-8.
We evaluated monoclonal antibodies to 3 cell surface and 3 intracellular antigens for their relative usefulness as markers to identify fetal cells in maternal blood.
With indirect immunocytochemical labeling techniques, antigen expression was studied in 52 fetal blood samples as a function of gestational age, fetal karyotype, the presence of multiple anomalies detectable on ultrasonography, and anemia.
A decline in the expression of these antigens as gestational age advanced was demonstrated. Samples from karyotypically abnormal fetuses, fetuses with multiple anomalies, and anemic fetuses showed an antigenic distribution that was immature for gestational age. In normal fetuses zeta globin and epsilon globin expression decreased after 12 to 14 weeks, potentially limiting the utility of these proteins as fetal cell markers in the isolation of fetal cells from maternal blood.
The results of this study demonstrate a fetal developmental hematologic profile that varies with gestational age and also with pathologic condition. Antibodies to the gamma chain of fetal hemoglobin and the transferrin receptor (CD71) are the most useful fetal cell-identifying reagents.
我们评估了针对3种细胞表面抗原和3种细胞内抗原的单克隆抗体作为识别母血中胎儿细胞标志物的相对效用。
采用间接免疫细胞化学标记技术，研究了52份胎儿血样中抗原表达与胎龄、胎儿核型、超声检查可检测到的多种异常情况以及贫血的关系。
随着胎龄增加，这些抗原的表达呈下降趋势。核型异常胎儿、有多种异常的胎儿以及贫血胎儿的样本显示出抗原分布，其胎龄不成熟。在正常胎儿中，ζ珠蛋白和ε珠蛋白表达在12至14周后下降，这可能限制了这些蛋白质作为从母血中分离胎儿细胞的胎儿细胞标志物的效用。
本研究结果表明胎儿发育血液学特征随胎龄以及病理状况而变化。针对胎儿血红蛋白γ链和转铁蛋白受体（CD71）的抗体是最有用的胎儿细胞识别试剂。
