Zheng Y L, Zhen D K, DeMaria M A, Berry S M, Wapner R J, Evans M I, Copeland D, Williams J M, Bianchi D W
Department of Pediatrics, New England Medical Center, Boston, MA 02111, USA.
Hum Genet. 1997 Jul;100(1):35-42. doi: 10.1007/s004390050462.
Fetal nucleated cells circulating in maternal peripheral blood are a noninvasive source of fetal DNA for prenatal genetic diagnoses. The successful isolation of fetal cells from maternal blood depends upon identification of differences between fetal and maternal cell surface antigen expression. To our best knowledge, a monoclonal antibody that binds only fetal blood cells has not yet been identified. We studied antigens recognized by six different monoclonal antibodies for their biologic expression on fetal blood cells as a function of gestational age, and compared their ability to bind fetal but not maternal cells. The results suggest a relationship between gestational age and nucleated cell surface antigen expression. The monoclonal antibodies FB3-2, H3-3, CD71 and 2-6B/6 are suitable reagents for first or early second trimester fetal cell isolation, although FB3-2 and H3-3 are more specific for fetal cells due to significantly lower expression of these antigens on maternal mononuclear cells. The observation that samples from fetuses with chromosome abnormalities or multiple structural anomalies express higher levels of these antigens indicates that these reagents will potentiate the detection of abnormal fetal cells in maternal blood samples.
循环于孕妇外周血中的胎儿有核细胞是用于产前基因诊断的无创胎儿DNA来源。从母血中成功分离胎儿细胞取决于识别胎儿和母体细胞表面抗原表达的差异。据我们所知，尚未鉴定出仅与胎儿血细胞结合的单克隆抗体。我们研究了六种不同单克隆抗体识别的抗原在胎儿血细胞上随胎龄的生物学表达，并比较了它们结合胎儿而非母体细胞的能力。结果提示胎龄与有核细胞表面抗原表达之间存在关联。单克隆抗体FB3-2、H3-3、CD71和2-6B/6是孕早期或孕中期早期分离胎儿细胞的合适试剂，尽管FB3-2和H3-3对胎儿细胞更具特异性，因为这些抗原在母体单核细胞上的表达显著较低。有染色体异常或多种结构异常的胎儿样本中这些抗原表达水平较高的观察结果表明，这些试剂将增强对母血样本中异常胎儿细胞的检测。
