Hidalgo-Sánchez M, Millet S, Simeone A, Alvarado-Mallart R M
INSERM Unité 106, Hôpital de la Salpêtrière, 47, Bld. de l'Hôpital, 75651, Paris CEDEX 13, France.
Mech Dev. 1999 Mar;81(1-2):175-8. doi: 10.1016/s0925-4773(98)00224-x.
The patterns of the Gbx2, Pax2, Wnt1, and Fgf8 gene expression were analyzed in the chick with respect to the caudal limit of the Otx2 anterior domain, taken as a landmark of the midbrain/hindbrain (MH) boundary. The Gbx2 anterior boundary is always concomitant with the Otx2 posterior boundary. The ring of Wnt1 expression is included within the Otx2 domain and Fgf8 transcripts included within the Gbx2 neuroepithelium. Pax2 expression is centred on the MH boundary with a double decreasing gradient. We propose a new nomenclature to differentiate the vesicles and constrictions observed in the avian MH domain at stage HH10 and HH20, based on the localization of the Gbx2/Otx2 common boundary.
以Otx2前区的尾侧界限作为中脑/后脑（MH）边界的标志，分析了鸡胚中Gbx2、Pax2、Wnt1和Fgf8基因的表达模式。Gbx2的前边界总是与Otx2的后边界相伴。Wnt1表达环包含在Otx2区域内，Fgf8转录本包含在Gbx2神经上皮内。Pax2表达以MH边界为中心，呈双递减梯度。我们基于Gbx2/Otx2共同边界的定位，提出了一种新的命名法，以区分在HH10和HH20阶段鸡胚MH区域观察到的囊泡和缩窄。
