Tul'skaia O L, Derenko M V, Maliarchuk B A
Institute of Biological Problems of the North, Russian Academy of Sciences, Magadan, Russia.
Genetika. 1999 Jan;35(1):17-21.
Variation in populations of sea otter Enhydra lutris lutris from Komandor islands and Kamchatka was studied in segment b1-b2 of cytochrome b gene and in the control region (main noncoding region) of mitochondrial DNA (mtDNA) by means of restriction analysis. The total sample size was 59 animals. Polymorphism was recorded only in the control mtDNA region for one restriction endonuclease (MboI). Frequencies of polymorphic variants were 64.7 and 35.3%, respectively. The low level of mtDNA variation in the sea otter populations examined is in good agreement with the published data on mtDNA polymorphism in populations of this species from north America. A discrepancy between the level of mtDNA variation and heterozygosity of protein-coding loci was demonstrated.
通过限制性分析,对来自科曼多尔群岛和堪察加半岛的海獭(Enhydra lutris lutris)种群,在细胞色素b基因的b1 - b2片段以及线粒体DNA(mtDNA)的控制区(主要非编码区)进行了研究。总样本量为59只动物。仅在控制mtDNA区域,针对一种限制性内切酶(MboI)记录到了多态性。多态性变体的频率分别为64.7%和35.3%。在所研究的海獭种群中,mtDNA变异水平较低,这与北美该物种种群中已发表的mtDNA多态性数据相符。同时还证明了mtDNA变异水平与蛋白质编码位点杂合性之间存在差异。