Radchenko O A, Derenko M V, Maliarchuk B A
Institute of Biological Problems of the Far North, Russian Academy of Sciences, Magadan, Russia.
Genetika. 2000 Jul;36(7):908-14.
Nucleotide sequence of the 307-bp fragment of the mitochondrial DNA cytochrome b gene was determined in representatives of the three species of the Salvelinus genus, specifically, dolly varden char (S. malma), taranetz char (S. taranetzi), and white-spotted char (S. leucomaenis). These results pointed to a high level of mitochondrial DNA (mtDNA) divergence between white-spotted char and dolly varden char, on the one hand, and taranetz char, on the other (the mean d value was 5.45%). However, the divergence between the dolly varden char and taranetz char was only 0.81%, which is comparable with the level of intraspecific divergence in the dolly varden char (d = 0.87%). It was shown that the dolly varden char mitochondrial gene pool contained DNA lineages differing from the main mtDNA pool at least in the taranetz char-specific mitochondrial lineages. One of these dolly varden char mtDNA lineages was characterized by the presence of the restriction endonuclease MspI-D variant of the cytochrome b gene. This lineage was widely distributed in the Chukotka populations but it was not detected in the Yana River (Okhotsk sea) populations. These findings suggest that dolly varden char has a more ancient evolutionary lineage, diverging from the common ancestor earlier than did taranetz char.
测定了红点鲑属三种鱼类（花羔红点鲑（S. malma）、塔拉内茨红点鲑（S. taranetzi）和白斑红点鲑（S. leucomaenis））线粒体DNA细胞色素b基因307 bp片段的核苷酸序列。这些结果表明，一方面白斑红点鲑与花羔红点鲑之间，另一方面与塔拉内茨红点鲑之间，线粒体DNA（mtDNA）存在高度差异（平均d值为5.45%）。然而，花羔红点鲑与塔拉内茨红点鲑之间的差异仅为0.81%，这与花羔红点鲑的种内差异水平（d = 0.87%）相当。结果表明，花羔红点鲑线粒体基因库中包含的DNA谱系至少在塔拉内茨红点鲑特有的线粒体谱系方面与主要的mtDNA库不同。这些花羔红点鲑mtDNA谱系之一的特征是细胞色素b基因存在限制性内切酶MspI-D变体。该谱系在楚科奇种群中广泛分布，但在亚纳河（鄂霍次克海）种群中未检测到。这些发现表明，花羔红点鲑有一个更古老的进化谱系，比塔拉内茨红点鲑更早地从共同祖先分化出来。
