Oneda K
Department of Internal Medicine, Saint Marianna University School of Medicine.
Arerugi. 1999 Jan;48(1):13-22.
The corticosteroid is effective for treating allergic inflammation. However, the mechanisms of the effectiveness are still unclarified. In this study, we examined whether dexamethasone, one of corticosteroids, can induce apoptosis in peripheral T cells from patients with asthma. Dexamethasone induced significantly greater apoptosis in T cells from patients with asthma than from normal subjects at 10(-4) M and 10(-5) M (p < 0.05). Apoptosis was assessed by flow cytometry, DNA fragmentation. By examining the function of T cell subsets, the augmentation of apoptosis was ascribed to CD4+HLADR+CD25+subsets. Elimination of HLADR+CD25+subsets from T cells caused a great reduction in IL-5 production and proliferation to mite on Day 3, suggesting that the subset might be primed by mite allergen. Dexamethasone may reduce allergen specific T cells through apoptosis, which is one of the mechanisms of effectiveness of dexamethasone in asthmatics.
皮质类固醇对治疗过敏性炎症有效。然而，其有效性的机制仍未阐明。在本研究中，我们检测了皮质类固醇之一的地塞米松是否能诱导哮喘患者外周血T细胞凋亡。在10^(-4)M和10^(-5)M浓度下，地塞米松诱导哮喘患者T细胞凋亡的程度显著高于正常受试者（p<0.05）。通过流式细胞术、DNA片段化评估凋亡情况。通过检测T细胞亚群的功能，发现凋亡增加归因于CD4+HLADR+CD25+亚群。从T细胞中去除HLADR+CD25+亚群会导致第3天IL-5产生和对螨虫的增殖大幅减少，这表明该亚群可能被螨虫变应原致敏。地塞米松可能通过凋亡减少变应原特异性T细胞，这是地塞米松对哮喘患者有效性的机制之一。
