Li Min, Song Li, Zhang Jian-Bo, Fang Jun, Li Lan
Department of Pediatrics, Sichuan Province People's Hospital, Sichuan Academy of Medical Sciences, Chengdu 610072, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2008 Aug;10(4):527-30.
To determine the changes of CD4+CD25+ regulatory T cells and the levels of IL-10 and TGF-beta1 in the mouse model of asthma and the effects of glucocorticoid inhalation on CD4+CD25+ regulatory T cells and IL-10 and TGF-beta1 levels.
Thirty BALB/c mice were randomly divided into three groups: asthma model, glucocorticoid treatment and control. Asthma was induced by OVA administration in the asthma model and the glucocorticoid treatment groups. The glucocorticoid treatment group received budesonide inhalation (0.8 mg/L) before the challenge of asthma. After 24 hrs of the last challenge, the lung tissues of middle lobe of the right lung were obtained for the observation of lung pathological changes. Peripheral anticoagulated blood and lung lymph cells suspension were collected. The percentage of CD4+CD25+ regulatory T cells in CD4+ T cells was detected by flow cytometry, and the levels of IL-10 and TGF-beta1 in plasma were measured using ELISA.
The percentage of CD4+CD25+ regulatory T cells in peripheral blood and lung lymph cells suspension in the asthma model group was significantly lower than the control group ( P<0.01). The glucocorticoid-treated asthma group showed an increased percentage of CD4+CD25+ regulatory T cells compared with the asthma model group (P<0.01) and a similar percentage of CD4+CD25+ regulatory T cells in peripheral blood and lung lymph cells suspension to the control group. Compared with the control group, plasma levels of IL-10 and TGF-beta1 decreased significantly in the asthma model group (P<0.01). After glucocorticoid treatment plasma IL-10 level increased significantly (P<0.01) and was similar to that in the control group, while plasma TGF-beta1 level remained lower than that in the control group.
The percentage of CD4+CD25+ regulatory T cells and the levels of IL-10 and TGF-beta1 decreased in asthmatic mice which might contribute to the pathogenesis of asthma. Glucocorticoid can increase the percentage of CD4+CD25+ regulatory T cells and the levels of IL-10 and thus provides protective effects against asthma. The changes of the percentage of CD4+CD25+ regulatory T cells in peripheral blood were consistent with those in the lung, suggesting that monitoring the changes of CD4+CD25+ regulatory T cells in peripheral blood may be useful to understand the changes of CD4+CD25+ regulatory T cells in the lung.
确定哮喘小鼠模型中CD4+CD25+调节性T细胞的变化以及白细胞介素-10（IL-10）和转化生长因子-β1（TGF-β1）的水平，以及吸入糖皮质激素对CD4+CD25+调节性T细胞和IL-10及TGF-β1水平的影响。
将30只BALB/c小鼠随机分为三组：哮喘模型组、糖皮质激素治疗组和对照组。哮喘模型组和糖皮质激素治疗组通过卵清蛋白（OVA）诱导哮喘。糖皮质激素治疗组在哮喘激发前吸入布地奈德（0.8 mg/L）。末次激发24小时后，获取右肺中叶肺组织观察肺病理变化。采集外周抗凝血和肺淋巴细胞悬液。采用流式细胞术检测CD4+T细胞中CD4+CD25+调节性T细胞的百分比，采用酶联免疫吸附测定法（ELISA）检测血浆中IL-10和TGF-β1的水平。
哮喘模型组外周血和肺淋巴细胞悬液中CD4+CD25+调节性T细胞的百分比显著低于对照组（P<0.01）。糖皮质激素治疗的哮喘组与哮喘模型组相比，CD4+CD25+调节性T细胞的百分比增加（P<0.01），外周血和肺淋巴细胞悬液中CD4+CD25+调节性T细胞的百分比与对照组相似。与对照组相比，哮喘模型组血浆中IL-10和TGF-β1水平显著降低（P<0.01）。糖皮质激素治疗后，血浆IL-10水平显著升高（P<0.01）且与对照组相似，而血浆TGF-β1水平仍低于对照组。
哮喘小鼠中CD4+CD25+调节性T细胞的百分比以及IL-10和TGF-β1水平降低，这可能有助于哮喘的发病机制。糖皮质激素可增加CD4+CD25+调节性T细胞的百分比和IL-10水平，从而对哮喘提供保护作用。外周血中CD4+CD25+调节性T细胞百分比的变化与肺中的变化一致，提示监测外周血中CD4+CD25+调节性T细胞的变化可能有助于了解肺中CD4+CD25+调节性T细胞的变化。
