Etlinger H M, Hodgins H O, Chiller J M
In Vitro. 1976 Aug;12(8):599-601. doi: 10.1007/BF02797445.
The addition of 10% fetal bovine serum to Leibovitz's L-15 culture medium resulted in marked growth of peripheral blood leukocytes from rainbow trout, Salmo gairdneri. Culture medium without serum or with 20% homologous serum did not induce substantial growth. In contrast to what has been reported by others, oxygenation of the culture medium was found not to be required for excellent cell growth.
在Leibovitz's L - 15培养基中添加10%胎牛血清，虹鳟（Salmo gairdneri）外周血白细胞显著生长。无血清或含20%同源血清的培养基未诱导出显著生长。与其他人的报道相反，发现培养基的氧合作用并非细胞良好生长所必需。
