Scott J C, McManus D P
Molecular Parasitology Unit, Australian Centre for International and Tropical Health and Nutrition, The University of Queensland, Post Office Royal Brisbane Hospital, Herston.
Int J Parasitol. 1999 Mar;29(3):437-44. doi: 10.1016/s0020-7519(98)00227-6.
A diverse range of organisms respond to a variety of chemical, physiological and temperature-associated stresses by a rapid and transient increase in the synthesis of heat shock proteins. We immunoscreened a Uni-ZAP XR cDNA library, prepared from mRNA isolated from the Philippine strain of the Asian bloodfluke, Schistosoma japonicum, using hyperimmune rabbit sera raised against soluble adult S. japonicum proteins. Six 70-kDa heat shock protein-encoding cDNA clones were identified which, upon further analysis, were separated into two distinct protein groups within the 70-kDa heat shock protein family, the 70-kDa heat shock proteins and the immunoglobulin heavy chain-binding proteins/glucose-related proteins (Grp78). A representative from both groups was fully sequenced and compared with homologous sequences available in the GenBank/EMBL database as the first stage in determining the role of their expression products in the regulation of S. japonicum development, in the induction of immunity, and whether they act as molecular chaperones capable of modulating the correct folding or repair of proteins within this species of schistosome.
各种各样的生物体通过热休克蛋白合成的快速短暂增加来应对各种化学、生理和与温度相关的应激。我们使用针对日本血吸虫可溶性成虫蛋白产生的超免疫兔血清，对从菲律宾株日本血吸虫分离的mRNA制备的Uni-ZAP XR cDNA文库进行了免疫筛选。鉴定出六个编码70 kDa热休克蛋白的cDNA克隆，进一步分析后，它们被分为70 kDa热休克蛋白家族内两个不同的蛋白质组，即70 kDa热休克蛋白和免疫球蛋白重链结合蛋白/葡萄糖相关蛋白（Grp78）。对两组中的一个代表进行了全序列测定，并与GenBank/EMBL数据库中可用的同源序列进行比较，作为确定其表达产物在日本血吸虫发育调控、免疫诱导中的作用以及它们是否作为能够调节该血吸虫物种内蛋白质正确折叠或修复的分子伴侣的第一步。
