Department of Parasitology, Xiangya School of Medicine, Central South University, 110 Xiangya Road, Changsha, 410078, People's Republic of China.
Parasitol Res. 2012 Jan;110(1):403-9. doi: 10.1007/s00436-011-2505-2. Epub 2011 Jul 8.
Two novel genes, SJCWL05 and SJCWL06, were harvested from screening of Schistosoma japonicum (S. japonicum) cercaria cDNA library by using pig sera vaccinated (VPS) with S. japonicum immature egg ws-vaccine (S. japonicum iEw). Prokaryotic recombinant plasmids pGEX-4T-1/SJCWL05 and pGEX-4T-1/SJCWL06 were constructed to analyze their immunogenicity, which was confirmed by SDS-PAGE and Western blotting. Two eukaryotic recombinant plasmids, pcDNA3/SJCWL05 and pcDNA3/SJCWL06, were constructed, and their ability to protect mice against challenge of S. japonicum was evaluated. All mice vaccinated with pcDNA3/SJCWL05 or pcDNA3/SJCWL06 developed ELISA-specific anti-S. japonicum SIEA (S. japonicum soluble immature egg antigens) antibody. Immunoprotection experiments showed that worms and liver eggs reduced 34.64% and 39.14% in the pcDNA3/SJCWL05 group and those reduced 27.17% and 27.95% in the pcDNA3/SJCWL06 group, respectively. The reduction rates of intestine and uterine eggs in female worms of both groups reached 39.45% and 38.5% as well as 30.02% and 28.7%, respectively. Results of our study suggest that novel genes, SJCWL05 and SJCWL06, are potential vaccine candidates against schistosomiasis japonica.
从日本血吸虫（S. japonicum）未成熟卵 ws 疫苗（S. japonicum iEw）免疫猪血清筛选日本血吸虫尾蚴 cDNA 文库中获得了两个新基因 SJCWL05 和 SJCWL06。构建了原核重组质粒 pGEX-4T-1/SJCWL05 和 pGEX-4T-1/SJCWL06 来分析其免疫原性，通过 SDS-PAGE 和 Western blot 进行了确认。构建了两个真核重组质粒 pcDNA3/SJCWL05 和 pcDNA3/SJCWL06，并评估了它们对小鼠抵抗日本血吸虫攻击的保护能力。所有接种 pcDNA3/SJCWL05 或 pcDNA3/SJCWL06 的小鼠均产生了 ELISA 特异性抗日本血吸虫可溶性未成熟卵抗原（S. japonicum SIEA）抗体。免疫保护实验表明，pcDNA3/SJCWL05 组的蠕虫和肝卵减少了 34.64%和 39.14%，pcDNA3/SJCWL06 组的蠕虫和肝卵减少了 27.17%和 27.95%。两组雌虫肠和子宫卵的减少率分别达到 39.45%和 38.5%以及 30.02%和 28.7%。我们的研究结果表明，新基因 SJCWL05 和 SJCWL06 是日本血吸虫病潜在的疫苗候选物。
