Thamsborg S M, Gray G D, Gill H S, Burgess S K, Lea J M
Division of Animal Science, University of New England, Armidale, NSW, Australia.
Int J Parasitol. 1999 Mar;29(3):451-8. doi: 10.1016/s0020-7519(98)00213-6.
The mammary glands of 103 pasture-reared non-lactating, non-pregnant Merino ewes were infused via the teat canal with antigens prepared from the nematode Haemonchus contortus, and the inflammatory response to infusion assessed by washing the gland of its contents after 24 h and 14 days. The ewes were of two genotypes: one with proven high levels of resistance to infection with the nematode H. contortus, the other random-bred animals with relative susceptibility to infection. On day 0 of a H. contortus infection, one gland of the subgroups of both genotypes was infused with the antigen preparation. At the same time, the other gland of the random-bred ewes was infused with sterile physiological saline. A third group of infected random-bred ewes was infused with only sterile physiological saline. Similar infusions were performed on other subgroups on days 12, 21 and 35 of infection, which was then terminated with anthelmintic. A fourth group of uninfected random-bred control ewes was given both infusions 35 days after the other groups were infected. Sheep of the resistant genotype had lower worm egg counts and smaller reductions in blood packed cell volumes from day 21 of infection. Infusion of antigen had no effect on the course of infection and no effect on the response of the other gland, which had been infused with saline alone. The dominant leukocyte response from the antigen-infused gland was eosinophilia. On all days of infusion, and after both 24 h and 14 days, eosinophil counts from the resistant genotype were higher than those from their random-bred counterparts. The sheep mammary gland provides a source of eosinophils whose number is related to host genotype and stage of infection and may provide a model for the investigation of cellular responses in mucosal immunity to nematode infections.
对103只在牧场饲养的非泌乳、未怀孕的美利奴母羊的乳腺，通过乳头管注入由捻转血矛线虫制备的抗原，并在24小时和14天后冲洗乳腺内容物来评估注入后的炎症反应。这些母羊有两种基因型：一种经证实对捻转血矛线虫感染具有高水平抗性，另一种是随机繁殖的、对感染相对易感的动物。在捻转血矛线虫感染的第0天，两种基因型亚组的一个乳腺注入抗原制剂。同时，随机繁殖母羊的另一个乳腺注入无菌生理盐水。第三组感染的随机繁殖母羊仅注入无菌生理盐水。在感染的第12天、21天和35天对其他亚组进行类似的注入，然后用驱虫药终止感染。第四组未感染的随机繁殖对照母羊在其他组感染35天后进行两次注入。抗性基因型的绵羊从感染第21天起虫卵计数较低，血液红细胞压积降低幅度较小。注入抗原对感染进程没有影响，对另一个仅注入生理盐水的乳腺的反应也没有影响。注入抗原的乳腺的主要白细胞反应是嗜酸性粒细胞增多。在所有注入日以及24小时和14天后，抗性基因型的嗜酸性粒细胞计数均高于其随机繁殖的对应物。绵羊乳腺提供了一个嗜酸性粒细胞来源，其数量与宿主基因型和感染阶段有关，可能为研究黏膜免疫中对线虫感染的细胞反应提供一个模型。
