Adams D B, Colditz I G
CSIRO Division of Animal Health, Pastoral Research Laboratory, Armidale, New South Wales, Australia.
Int J Parasitol. 1991 Oct;21(6):631-9. doi: 10.1016/0020-7519(91)90074-h.
Cellular exudates induced by infusion with helminth antigens were examined in non-lactating mammary glands of ewes immune to infection with the abomasal nematode, Haemonchus contortus. Secondary immunological responsiveness was expressed in two ways. Firstly, antigens from adult H. contortus elicited larger eosinophil-rich cellular exudates in immune compared to non-immune ewes. In this situation, secondary responsiveness in the mammary gland must have been generated through abomasal infection with the parasite. Secondly, repeated infusion with the antigens from adult H. contortus increased the size of cellular exudates in both immune and non-immune ewes. Eosinophils predominated but numbers of macrophages and lymphocytes were also increased. In this second situation, secondary responsiveness must have been either supplemented in immune ewes or derived completely in non-immune ewes by contact with helminth antigens through the mammary gland. The helminth antigens which induce eosinophil exudates in the mammary gland may not be potently protective against H. contortus. Furthermore, eosinophil exudation may not be an in vivo correlate of immunity which is directly useful for discriminating protective antigens and applicable to vaccine development. Infusion with antigens from adult forms of either H. contortus or Trichostrongylus colubriformis elicited cellular exudates equally well in immune ewes primed by infusion with H. contortus adult antigens 7 days beforehand. In addition, antigens from infective larvae of H. contortus elicited cellular exudates more potently than antigens from adult worms. However, vaccination with irradiated larvae has shown that species-specific protective immunity for H. contortus is stronger than cross-protective immunity conferred by T. colubriformis.(ABSTRACT TRUNCATED AT 250 WORDS)
在对皱胃线虫捻转血矛线虫感染具有免疫力的母羊的非泌乳乳腺中，检查了注入蠕虫抗原诱导的细胞渗出物。二次免疫反应以两种方式表现出来。首先，与未免疫的母羊相比，来自成年捻转血矛线虫的抗原在免疫母羊中引发了更大的富含嗜酸性粒细胞的细胞渗出物。在这种情况下，乳腺中的二次反应一定是通过寄生虫的皱胃感染产生的。其次，反复注入来自成年捻转血矛线虫的抗原会增加免疫和未免疫母羊中细胞渗出物的大小。嗜酸性粒细胞占主导，但巨噬细胞和淋巴细胞的数量也增加了。在第二种情况下，二次反应一定是在免疫母羊中得到补充，或者在未免疫母羊中完全通过乳腺与蠕虫抗原接触而产生。在乳腺中诱导嗜酸性粒细胞渗出物的蠕虫抗原可能对捻转血矛线虫没有很强的保护作用。此外，嗜酸性粒细胞渗出可能不是免疫的体内相关指标，直接用于区分保护性抗原并应用于疫苗开发。向事先7天注入捻转血矛线虫成年抗原致敏的免疫母羊中注入来自成年捻转血矛线虫或蛇形毛圆线虫的抗原，同样能很好地引发细胞渗出物。此外，来自捻转血矛线虫感染性幼虫的抗原比来自成虫的抗原更有效地引发细胞渗出物。然而，用辐照幼虫进行疫苗接种表明，捻转血矛线虫的种特异性保护性免疫比蛇形毛圆线虫赋予的交叉保护性免疫更强。（摘要截断于250字）
