Johnsson R, Vuopio P
Acta Haematol. 1978;60(6):329-40. doi: 10.1159/000207732.
A method for studying red cell flexibility as reflected by red cell rigidity and fragility is described. Using an infusion pump, suspended red cells are filtered through a polycarbonate membrane with 3 micron pores. The filtration pressure, which is continuously monitored, is considered to reflect red cell rigidity. The hemoglobin released by the disruption of red cells passing through the membrane is regarded as an indirect measure of red cell fragility. In all of the patients with hereditary spherocytosis studied and in some of their symptomless relatives, the decreased flexibility observed was associable with increased rigidity of the red cell. In vitro effects of chlorpromazine and vinca alkaloid on red cell flexibility were also studied. High concentrations of chlorpromazine and vinblastine induced formation of spherocytes displaying altered cell flexibility.
本文描述了一种通过红细胞刚性和脆性来研究红细胞柔韧性的方法。使用输液泵，将悬浮的红细胞通过具有3微米孔径的聚碳酸酯膜进行过滤。持续监测的过滤压力被认为反映了红细胞的刚性。通过膜的红细胞破裂所释放的血红蛋白被视为红细胞脆性的间接指标。在所有研究的遗传性球形红细胞增多症患者及其一些无症状亲属中，观察到的柔韧性降低与红细胞刚性增加有关。还研究了氯丙嗪和长春花生物碱对红细胞柔韧性的体外作用。高浓度的氯丙嗪和长春花碱诱导形成了显示细胞柔韧性改变的球形红细胞。
