通过Percoll密度梯度离心法显示的遗传性球形红细胞增多症中红细胞异常老化模式。

Abnormal pattern of erythrocyte ageing in hereditary spherocytosis as shown by Percoll density gradient centrifugation.

作者信息

Bell C M, Parker A C, Maddy A H

出版信息

Clin Chim Acta. 1984 Sep 15;142(1):91-102. doi: 10.1016/0009-8981(84)90104-9.

Abstract

Erythrocytes from individuals with Hereditary Spherocytosis (HS) were fractionated on Percoll density gradients. The osmotic fragility, mean cell volume, mean cell haemoglobin concentration, reticulocyte count and cell morphology of the fractions showed that the gradients separate the erythrocytes according to age, the older small spherocytes appearing in the dense fractions. Comparison of the well established parameters of erythrocyte ageing, pyruvate kinase activity, acetylcholinesterase activity and cell potassium content in normal and HS erythrocyte fractions of similar age revealed abnormalities in the senescence of HS erythrocytes both before and after splenectomy.

摘要

对患有遗传性球形红细胞增多症（HS）个体的红细胞在Percoll密度梯度上进行分级分离。各组分的渗透脆性、平均细胞体积、平均细胞血红蛋白浓度、网织红细胞计数和细胞形态表明，该梯度可根据年龄分离红细胞，较老的小球形红细胞出现在高密度组分中。对年龄相似的正常和HS红细胞组分中红细胞衰老的成熟参数（丙酮酸激酶活性、乙酰胆碱酯酶活性和细胞钾含量）进行比较，发现在脾切除术前和术后HS红细胞的衰老均存在异常。