Lisowska K, Długoński J
Department of Industrial Microbiology, University of Lódź, Poland.
J Basic Microbiol. 1999;39(2):117-25.
Nine fungal strains showing ability of cortexolone hydroxylation to epicortisol and/or cortisol were screened in this work for anthracene and phenanthrene elimination (250 mg/l). All of the strains (Cylindrocladium simplex IM 2358, C. simplex IM 2358/650, Monosporium olivaceum IM 484, Curvularia lunata IM 2901, C. lunata IM 2901/366, C. tuberculata IM 4417, Cunninghamella elegans IM 1785, C. elegans IM 1785/21Gp, C. elegans IM 1785/10Gi) significantly removed anthracene and phenanthrene. During incubation with anthracene formation of intermediate products was observed. The amount of the main intermediate product, identified as 9, 10-anthraquinone, was not greater than 22.2% of the anthracene introduced to the fungal cultures. C. elegans IM 1785/21Gp was the best degrader of both anthracene and phenanthrene, removing 81.6 and 99.4% of these compounds after 7 days, respectively. Phenanthrene removal by C. elegans IM 1785/21Gp was preceded by PAHs accumulation in mycelium and growth inhibition. Elimination of phenanthrene started after one day of incubation and was related to the fungus growth.
在本研究中，筛选了九株具有将皮质醇羟基化为表皮质醇和/或皮质醇能力的真菌菌株，用于去除蒽和菲（250毫克/升）。所有菌株（简单柱孢霉IM 2358、简单柱孢霉IM 2358/650、橄榄单孢霉IM 484、新月弯孢霉IM 2901、新月弯孢霉IM 2901/366、瘤状弯孢霉IM 4417、雅致小克银汉霉IM 1785、雅致小克银汉霉IM 1785/21Gp、雅致小克银汉霉IM 1785/10Gi）都能显著去除蒽和菲。在与蒽一起培养的过程中，观察到了中间产物的形成。鉴定为9,10-蒽醌的主要中间产物的量不超过引入真菌培养物中的蒽的22.2%。雅致小克银汉霉IM 1785/21Gp是蒽和菲的最佳降解菌株，7天后分别去除了这些化合物的81.6%和99.4%。雅致小克银汉霉IM 1785/21Gp去除菲之前，多环芳烃在菌丝体中积累并抑制生长。培养一天后开始去除菲，这与真菌生长有关。
