Lisowska Katarzyna, Długoński Jerzy, Freeman James P, Cerniglia Carl E
Department of Industrial Microbiology and Biotechnology, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland.
Chemosphere. 2006 Aug;64(9):1499-506. doi: 10.1016/j.chemosphere.2005.12.066. Epub 2006 Feb 28.
The metabolism of phenanthrene and the mammalian corticosteroid hormone cortexolone by the fungus Cunninghamella elegans was studied. The amounts of the cortexolone transformation products, cortisol and epicortisol, were affected by the presence of phenanthrene. Approximately 40% more cortisol was produced by C. elegans in cultures with phenanthrene. In contrast, epicortisol formation decreased. C. elegans transformed phenanthrene to phenanthrene trans-1,2-,3,4-, and 9,10-dihydrodiols, phenols, diphenols (diols) and glucoside conjugates of 1-, 2-, 3-, 4-, and 9-phenanthrols. Almost all of the phenanthrene initially added was metabolized to ethyl acetate extractable metabolites. In the mycelia and culture medium extracts, phenanthrol glucosides represented 80% and 94% of the total metabolites, respectively. The major metabolite was the glucoside conjugate of 1-phenanthrol. The presence of cortexolone affected the biodegradation of phenanthrene by decreasing the amounts of phenanthrene metabolites compared to control cultures.
研究了真菌雅致小克银汉霉对菲和哺乳动物皮质类固醇激素皮质醇的代谢。皮质醇转化产物皮质醇和表皮质醇的量受菲的存在影响。在含有菲的培养物中，雅致小克银汉霉产生的皮质醇大约多40%。相比之下，表皮质醇的形成减少。雅致小克银汉霉将菲转化为菲反式-1,2-、3,4-和9,10-二氢二醇、酚类、二酚（二醇）以及1-、2-、3-、4-和9-菲醇的葡萄糖苷缀合物。最初添加的几乎所有菲都被代谢为可被乙酸乙酯萃取的代谢物。在菌丝体和培养基提取物中，菲醇葡萄糖苷分别占总代谢物的80%和94%。主要代谢物是1-菲醇的葡萄糖苷缀合物。与对照培养物相比，皮质醇的存在通过减少菲代谢物的量来影响菲的生物降解。
